Many biologically active small‐molecule natural products produced by microorganisms
derive their activities from sugar substituents. Changing the structures of these sugars can …

Glycosylation of small molecules like specialized (secondary) metabolites has a profound
impact on their solubility, stability or bioactivity, making glycosides attractive compounds as …

Quantitative proteome profiling using stable isotope protein tagging and automated tandem
mass spectrometry (MS/MS) is an emerging technology with great potential for the functional …

Membrane proteins mediate a variety of cellular responses to extracellular signals. Although
membrane proteins are studied intensively for their values as disease biomarkers and …

Protein glycosylation is a common post-translational modification and has been increasingly
recognized as one of the most prominent biochemical alterations associated with malignant …

Glycoprotein changes occur in not only protein abundance but also the occupancy of each
glycosylation site by different glycoforms during biological or pathological processes. Recent …

This review focuses on techniques for quantification and identification in proteomics by
stable isotope coding. Methods are examined for analyzing expression, post-translational …

Erythropoietin (EPO) from sera obtained from anemic patients was successfully isolated
using magnetic beads coated with a human EPO (hEPO)–specific antibody. Human serum …

In this article, we present an overview of the different strategies for sample preparation for
identification by mass spectrometry (MS) of biomarkers from serum and/or plasma. We …

A strategy is developed in this study for identifying sialylated glycoprotein markers in human
cancer serum. This method consists of three steps: lectin affinity selection, a liquid …