The epigenome involves a complex set of cellular processes governing genomic activity.
Dissecting this complexity necessitates the development of tools capable of specifically …

The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for
sequence-specific regulation of gene expression. This Review describes the development of …

Epigenome editing with the CRISPR (clustered, regularly interspaced, short palindromic
repeats)-Cas9 platform is a promising technology for modulating gene expression to direct …

Large genome-mapping consortia and thousands of genome-wide association studies have
identified non-protein-coding elements in the genome as having a central role in various …

Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and
thoroughly test the cell type–specific function. Here, we use a nuclease-deficient Cas9 …

TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …

Embryonic stem cell (ESC) culture conditions are important for maintaining long-term self-
renewal, and they influence cellular pluripotency state. Here, we report single cell RNA …

Gene regulation is a complex and tightly controlled process that defines cell identity, health
and disease, and response to pharmacologic and environmental signals. Recently …