Macromolecular crowding effects on protein dynamics

N Das, T Khan, B Halder, S Ghosh, P Sen - International Journal of …, 2024 - Elsevier
Macromolecular crowding experiments bridge the gap between in-vivo and in-vitro studies
by mimicking some of the cellular complexities like high viscosity and limited space, while …

Role of Associated Water Dynamics on Protein Stability and Activity in Crowded Milieu

T Khan, B Halder, N Das, P Sen - The Journal of Physical …, 2024 - ACS Publications
Macromolecular crowding bridges in vivo and in vitro studies by simulating cellular
complexities such as high viscosity and limited space while maintaining the experimental …

Microscopic Heterogeneity Driven by Molecular Aggregation and Water Dynamics in Aqueous Osmolyte Solutions

J Seo, R Singh, JH Choi - Journal of Chemical Information and …, 2025 - ACS Publications
Water dynamics are investigated in binary osmolyte–water mixtures, exhibiting a
microscopic heterogeneity driven by molecular aggregation, on the basis of molecular …

Critical Role of Water beyond the Media to Maintain Protein Stability and Activity in Hydrated Deep Eutectic Solvent

T Khan, N Das, S Bhowmik, KS Negi… - The Journal of Physical …, 2024 - ACS Publications
Hydrated deep eutectic solvents (DESs) are recognized for their potential in biocatalysis due
to their tunability, biocompatibility, greenness, and ability to keep protein stable and active …

Molecular dynamics and solvation structures of the β-glucosidase from Humicola insolens (BGHI) in aqueous solutions containing glucose

FC Ramos, L Martínez - International Journal of Biological Macromolecules, 2025 - Elsevier
The β-glucosidase enzyme is a glycosyl hydrolase that breaks down the β-1, 4 linkage of
cellobiose. It is inhibited by glucose at high concentrations due to competitive inhibition …

Rotational Dynamics of Water near Osmolytes by Molecular Dynamics Simulations

Y Higuchi, MA Saleh, T Anada, M Tanaka… - The Journal of …, 2024 - ACS Publications
The behavior of water molecules around organic molecules has attracted considerable
attention as a crucial factor influencing the properties and functions of soft matter and …

Tracking heterogenous protein aggregation at nanoscale through fluorescence correlation spectroscopy

B Halder, S Ghosh, T Khan, S Pal… - Photochemistry and …, 2024 - Wiley Online Library
Various biophysical techniques have been extensively employed to study protein
aggregation due to its significance. Traditionally, these methods detect aggregation at …

Amino acids and glycine derivatives differently affect refolding of mesophilic and thermophilic like α-amylases: implications in protein refolding and aggregation

A Ahmad, P Joshi, R Mishra - Journal of Biomolecular Structure and …, 2024 - Taylor & Francis
Abstract α-amylases are industrially important enzymes which are used in different starch-
based industries. They are adapted to different environmental conditions like extremes of …

Assessing the Effect of Deep Eutectic Solvents on α‐Chymotrypsin Thermal Stability and Activity

NF Gajardo‐Parra, E Cea‐Klapp, A Chandra… - … - Wiley Online Library
Optimizing the liquid reaction phase holds significant potential for enhancing the efficiency
of biocatalytic processes since it determines reaction equilibrium and kinetics. This study …

Adaptation to Drought Stress in Cereals: A Molecular Perspective

C Ikan, FE Soussani, S Toubali, N El Mazouni… - Plant Stress … - taylorfrancis.com
Accelerated climate change and a growing population worldwide have caused an increase
in abiotic stress and a reduction in agricultural productivity. Drought stress (DS) can …