With reference to reference genes: a systematic review of endogenous controls in gene expression studies

JR Chapman, J Waldenström - PloS one, 2015 - journals.plos.org
The choice of reference genes that are stably expressed amongst treatment groups is a
crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have …

What determines growth potential and juvenile quality of farmed fish species?

LMP Valente, KA Moutou, LEC Conceicao… - Reviews in …, 2013 - Wiley Online Library
Enhanced production of high quality and healthy fry is a key target for a successful and
competitive expansion of the aquaculture industry. Although large quantities of fish larvae …

Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR

R Hu, C Fan, H Li, Q Zhang, YF Fu - BMC molecular biology, 2009 - Springer
Background Real-time quantitative reverse transcription PCR (RT-qPCR) data needs to be
normalized for its proper interpretation. Housekeeping genes are routinely employed for this …

Mechanical stimuli differentially control stem cell behavior: morphology, proliferation, and differentiation

TM Maul, DW Chew, A Nieponice, DA Vorp - Biomechanics and modeling …, 2011 - Springer
Mesenchymal stem cell (MSC) therapy has demonstrated applications in vascular
regenerative medicine. Although blood vessels exist in a mechanically dynamic …

[HTML][HTML] Reference gene selection for qPCR analysis during somatic embryogenesis in longan tree

YL Lin, ZX Lai - Plant Science, 2010 - Elsevier
Real-time reverse transcriptase PCR is a powerful tool to investigate relevant changes in
gene expression during plant somatic embryogenesis (SE); however, this method lacks …

Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)

GM Shen, HB Jiang, XN Wang, JJ Wang - BMC molecular biology, 2010 - Springer
Background Quantitative real-time reverse transcriptase PCR (RT-qPCR) has been widely
used for quantification of mRNA as a way to determine key genes involved in different …

[HTML][HTML] Application of multi-species of Bacillus in sea bream larviculture

MA Avella, G Gioacchini, O Decamp, P Makridis… - Aquaculture, 2010 - Elsevier
To date, probiotics can be considered a valid alternative to the use of antibiotics in
aquaculture and in particular, in fish larviculture, to prevent high mortality and to improve …

[HTML][HTML] PCB and OCP accumulation and evidence of hepatic alteration in the Atlantic bluefin tuna, T. thynnus, from the Mediterranean Sea

M Maisano, T Cappello, S Oliva, A Natalotto… - Marine Environmental …, 2016 - Elsevier
Persistent organic pollutants (POPs) are known to act as “obesogens”, being fat-soluble and
affecting lipid metabolism. The Atlantic bluefin tuna, Thunnus thynnus, are top pelagic …

[HTML][HTML] Molecular evolution of zebrafish dnmt3 genes and thermal plasticity of their expression during embryonic development

C Campos, LMP Valente, JMO Fernandes - Gene, 2012 - Elsevier
DNA reprogramming by DNA (cytosine-5)-methyltransferases (dnmts) after fertilisation is a
dynamic mechanism that is essential for early development. Amongst the three types of dnmt …

[HTML][HTML] Plant protein blends in diets for Senegalese sole affect skeletal muscle growth, flesh texture and the expression of related genes

LMP Valente, EM Cabral, V Sousa, LM Cunha… - Aquaculture, 2016 - Elsevier
Skeletal muscle growth and flesh quality were evaluated in Senegalese sole fed plant
protein (PP) diets. A control fish meal-based diet (FM) was compared with three …