Alternative splicing of pre-mRNA increases genetic diversity, and recent studies estimate
that most human multiexon genes are alternatively spliced. If this process is not highly …

Mucus pathology in cystic fibrosis (CF) has been known for as long as the disease has been
recognized and is sometimes called mucoviscidosis. The disease is marked by mucus …

The cloning of foreign DNA in Escherichia coli episomes is a cornerstone of molecular
biology. The pioneering work in the early 1970s, using DNA ligases to paste DNA into …

In 1993, several groups, working independently, reported the successful generation of
transgenic mice with yeast artificial chromosomes (YACs) using standard techniques. The …

The cystic fibrosis (CF) field is the beneficiary of five species of animal models that lack
functional cystic fibrosis transmembrane conductance regulator (CFTR) channel. These …

Cystic fibrosis (CF) is caused by mutations in the gene encoding the cystic fibrosis
transmembrane conductance regulator (CFTR) protein. Since~ 5% of all mutant CF alleles …

Mutations resulting in embryonic or early postnatal lethality could mask the activities of any
gene in unrelated and temporally distinct developmental pathways. Targeted inactivation of …

Clinical trials in cystic fibrosis (CF) patients established proof-of-principle for transfer of the
wild-type cystic fibrosis transmembrane conductance regulator (CFTR) gene to airway …

In recent years, mouse models for human metabolic diseases have become commonplace
because the information gained from in vivo study of biochemical pathways is invaluable …

The cystic fibrosis transmembrane conductance regulator gene (CFTR) shows clear
temporal and developmental regulation of its expression. However, there are few well …