In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

The combination of confocal laser-scanning microscopy (CLSM) and fluorescence
fluctuation spectroscopy (FFS) is a powerful tool in studying fast, sub-resolution …

Cells crucially rely on the interactions of biomolecules at their plasma membrane to maintain
homeostasis. Yet, a methodology to systematically quantify biomolecular organisation …

The single-photon timing and sensitivity performance and the imaging ability of
asynchronous-readout single-photon avalanche diode (SPAD) array detectors have opened …

Cellular function is reliant on the dynamic interplay between the plasma membrane and the
actin cytoskeleton. This critical relationship is of particular importance in immune cells …

Plasma membrane fluidity is an important phenotypic feature that regulates the diffusion,
function, and folding of transmembrane and membrane-associated proteins. In bacterial …

The diffusion and reorganization of phospholipids and membrane-associated proteins are
fundamental for cellular function. Fluorescence cross-correlation spectroscopy (FCCS) …

Cellular functions rely on proper actions of organelles such as peroxisomes. These
organelles rely on the import of proteins from the cytosol. The peroxisomal import receptor …

Endocytosis, apart from providing a mechanism for cells to take up nutrients, is the principal
route of entry for many nanomedicines. The evaluation of therapeutic delivery efficiency …

Advanced fluorescence microscopy studies require specific and monovalent molecular
labeling with bright and photostable fluorophores. This necessity led to the widespread use …