Increasing numbers of studies seek to characterize the different cellular sub-populations present in mammalian tissues. The techniques “Isolation of Nuclei Tagged in Specific Cell …
The chromatin landscape defines cellular identity in multicellular organisms with unique patterns of DNA accessibility and histone marks decorating the genome of each cell type …
A genetically modified biotin ligase BirA (Arg118Gly), termed BioID (BirA*), can be induced to add a reactive form of biotin to proximate proteins in the cytosol and nucleus. The …