Aqueous two-phase system (ATPS): an overview and advances in its applications

M Iqbal, Y Tao, S Xie, Y Zhu, D Chen, X Wang… - Biological procedures …, 2016 - Springer
Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained
an interest because of great potential for the extraction, separation, purification and …

Recent advances in large-scale production of monoclonal antibodies and related proteins

AA Shukla, J Thömmes - Trends in biotechnology, 2010 - cell.com
The rapid development of high-yielding and robust manufacturing processes for monoclonal
antibodies is an area of significant focus in the biopharmaceutical landscape. Advances in …

Deep eutectic solvents as novel extraction media for protein partitioning

Q Zeng, Y Wang, Y Huang, X Ding, J Chen, K Xu - Analyst, 2014 - pubs.rsc.org
Four kinds of green deep eutectic solvent (DES) were synthesized, including choline
chloride (ChCl)–urea, tetramethylammonium chloride (TMACl)–urea, tetrapropylammonium …

Technology trends in antibody purification

P Gagnon - Journal of chromatography A, 2012 - Elsevier
This article reviews technology trends in antibody purification. Section 1 discusses non-
chromatography methods, including precipitation, liquid–liquid extraction, and high …

Aqueous two-phase systems based on ionic liquids and deep eutectic solvents as a tool for the recovery of non-protein bioactive compounds—a review

FS Buarque, GV Gautério, MAZ Coelho, AC Lemes… - Processes, 2022 - mdpi.com
Aqueous two-phase systems (ATPS) based on ionic liquids (IL) and deep eutectic solvents
(DES) are ecofriendly choices and can be used to selectively separate compounds of …

Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing

AM Azevedo, PAJ Rosa, IF Ferreira… - Trends in …, 2009 - cell.com
The therapeutic use of proteins has created an increasing demand for feasible and
economical methods for both up-and downstream processes. However, whereas upstream …

Partitioning in aqueous two‐phase systems: Analysis of strengths, weaknesses, opportunities and threats

RRG Soares, AM Azevedo, JM Van Alstine… - Biotechnology …, 2015 - Wiley Online Library
For half a century aqueous two‐phase systems (ATPSs) have been applied for the extraction
and purification of biomolecules. In spite of their simplicity, selectivity, and relatively low cost …

Aqueous two-phase affinity partitioning systems: current applications and trends

F Ruiz-Ruiz, J Benavides, O Aguilar… - … of Chromatography A, 2012 - Elsevier
Aqueous two-phase systems (ATPS) have been studied and used for product recovery and
purification from diverse biological sources. ATPS are characterized by their versatility, easy …

Ionic liquids as adjuvants for the tailored extraction of biomolecules in aqueous biphasic systems

JFB Pereira, ÁS Lima, MG Freire, JAP Coutinho - Green Chemistry, 2010 - pubs.rsc.org
The potential use of ionic liquids (ILs) as adjuvants in typical polymer-salt aqueous systems
for the separation and purification of vital biomolecules is investigated. An innovative study …

Ionic liquids as additives to enhance the extraction of antioxidants in aqueous two-phase systems

MR Almeida, H Passos, MM Pereira, ÁS Lima… - Separation and …, 2014 - Elsevier
Aqueous two-phase systems (ATPS) have been proposed as an alternative technique for
the extraction, separation and/or purification of diverse biomolecules. Besides the typical …