Asymmetric bulges within hairpin RNA transgenes influence small RNA size, secondary siRNA production and viral defence
D Zhang, D Jue, N Smith, C Zhong… - Nucleic Acids …, 2024 - academic.oup.com
Small RNAs (sRNAs) are essential for normal plant development and range in size classes
of 21–24 nucleotides. The 22nt small interfering RNAs (siRNAs) and miRNAs are processed …
of 21–24 nucleotides. The 22nt small interfering RNAs (siRNAs) and miRNAs are processed …
DEMETER plays a role in DNA demethylation and disease response in somatic tissues of Arabidopsis
ABSTRACT DNA demethylases function in conjunction with DNA methyltransferases to
modulate genomic DNA methylation levels in plants. The Arabidopsis genome contains four …
modulate genomic DNA methylation levels in plants. The Arabidopsis genome contains four …
Ectopic Expression of PtoMYB74 in Poplar and Arabidopsis Promotes Secondary Cell Wall Formation
C Li, X Ma, H Yu, Y Fu, K Luo - Frontiers in Plant Science, 2018 - frontiersin.org
In vascular plants, R2R3-MYB transcription factors are important regulators of secondary cell
wall formation. Although 192 annotated R2R3 MYB genes were identified in the poplar …
wall formation. Although 192 annotated R2R3 MYB genes were identified in the poplar …
Hairpin RNAs derived from RNA polymerase II and polymerase III promoter-directed transgenes are processed differently in plants
RNA polymerase III (Pol III) as well as Pol II (35S) promoters are able to drive hairpin RNA
(hpRNA) expression and induce target gene silencing in plants. siRNAs of 21 nt are the …
(hpRNA) expression and induce target gene silencing in plants. siRNAs of 21 nt are the …
The multifaceted roles of R2R3 transcription factor HlMYB7 in the regulation of flavonoid and bitter acids biosynthesis, development and biotic stress tolerance in hop …
Hop (Humulus lupulus) biosynthesizes the highly economically valuable secondary
metabolites, which include flavonoids, bitter acids, polyphenols and essential oils. These …
metabolites, which include flavonoids, bitter acids, polyphenols and essential oils. These …
Dissociation (Ds) constructs, mapped Ds launch pads and a transiently-expressed transposase system suitable for localized insertional mutagenesis in rice
We have developed a transiently-expressed transposase (TET)-mediated Dissociation (D s)
insertional mutagenesis system for generating stable insertion lines in rice which will allow …
insertional mutagenesis system for generating stable insertion lines in rice which will allow …
Satellite RNAs interfere with the function of viral RNA silencing suppressors
WX Shen, PCK Au, BJ Shi, NA Smith… - Frontiers in Plant …, 2015 - frontiersin.org
Viral satellite RNAs (satRNAs) are small subviral RNAs and depend on the helper virus for
replication and spread. satRNAs can attenuate helper virus-induced symptoms, the …
replication and spread. satRNAs can attenuate helper virus-induced symptoms, the …
Transgene expression and transgene-induced silencing in diploid and autotetraploid Arabidopsis
Previous studies have suggested that transgene expression in plants can be affected by
ploidy. Here we show that three different transgenes, a reporter transgene, an antisense …
ploidy. Here we show that three different transgenes, a reporter transgene, an antisense …
Nicotiana Small RNA Sequences Support a Host Genome Origin of Cucumber Mosaic Virus Satellite RNA
K Zahid, JH Zhao, NA Smith, U Schumann… - PLoS …, 2015 - journals.plos.org
Satellite RNAs (satRNAs) are small noncoding subviral RNA pathogens in plants that
depend on helper viruses for replication and spread. Despite many decades of research, the …
depend on helper viruses for replication and spread. Despite many decades of research, the …
Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants
D Zhang, C Zhong, NA Smith, R de Feyter… - Nature …, 2022 - nature.com
Hairpin RNA (hpRNA) transgenes are the most successful RNA interference (RNAi) method
in plants. Here, we show that hpRNA transgenes are invariably methylated in the inverted …
in plants. Here, we show that hpRNA transgenes are invariably methylated in the inverted …