Metabolomics-based engineering for biofuel and bio-based chemical production in microalgae and cyanobacteria: A review
Metabolomics, an essential tool in modern synthetic biology based on the design-build-test-
learn platform, is useful for obtaining a detailed understanding of cellular metabolic …
learn platform, is useful for obtaining a detailed understanding of cellular metabolic …
Tracking the metabolic pulse of plant lipid production with isotopic labeling and flux analyses: past, present and future
Metabolism is comprised of networks of chemical transformations, organized into integrated
biochemical pathways that are the basis of cellular operation, and function to sustain life …
biochemical pathways that are the basis of cellular operation, and function to sustain life …
INCA 2.0: A tool for integrated, dynamic modeling of NMR-and MS-based isotopomer measurements and rigorous metabolic flux analysis
Metabolic flux analysis (MFA) combines experimental measurements and computational
modeling to determine biochemical reaction rates in live biological systems. Advancements …
modeling to determine biochemical reaction rates in live biological systems. Advancements …
Isotopically nonstationary 13C flux analysis of cyanobacterial isobutyraldehyde production
We applied isotopically nonstationary 13 C metabolic flux analysis (INST-MFA) to compare
the pathway fluxes of wild-type (WT) Synechococcus elongatus PCC 7942 to an engineered …
the pathway fluxes of wild-type (WT) Synechococcus elongatus PCC 7942 to an engineered …
Metabolic engineering tools in model cyanobacteria
AL Carroll, AE Case, A Zhang, S Atsumi - Metabolic engineering, 2018 - Elsevier
Developing sustainable routes for producing chemicals and fuels is one of the most
important challenges in metabolic engineering. Photoautotrophic hosts are particularly …
important challenges in metabolic engineering. Photoautotrophic hosts are particularly …
Isotopically nonstationary metabolic flux analysis (INST-MFA): putting theory into practice
Typically, 13 C flux analysis relies on assumptions of both metabolic and isotopic steady
state. If metabolism is steady but isotope labeling is not allowed to fully equilibrate …
state. If metabolism is steady but isotope labeling is not allowed to fully equilibrate …
Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942
Isotopically nonstationary metabolic flux analysis (INST-MFA) provides a versatile platform to
quantitatively assess in vivo metabolic activities of autotrophic systems. By applying INST …
quantitatively assess in vivo metabolic activities of autotrophic systems. By applying INST …
Accelerating the development of a sustainable bioenergy portfolio through stable isotopes
E Blanc‐Betes, N Gomez‐Casanovas… - GCB …, 2023 - Wiley Online Library
Bioenergy could help limit global warming to 2° C above pre‐industrial levels while
supplying almost a fourth of the world's renewable energy needs by 2050. However, the …
supplying almost a fourth of the world's renewable energy needs by 2050. However, the …
Overexpression of a diacylglycerol acyltransferase gene in Phaeodactylum tricornutum directs carbon towards lipid biosynthesis
J Dinamarca, O Levitan, GK Kumaraswamy… - Journal of …, 2017 - Wiley Online Library
Under nutrient deplete conditions, diatoms accumulate between 15% to 25% of their dry
weight as lipids, primarily as triacylglycerols (TAG s). As in most eukaryotes, these …
weight as lipids, primarily as triacylglycerols (TAG s). As in most eukaryotes, these …
Metabolic Flux Analysis of the Synechocystis sp. PCC 6803 ΔnrtABCD Mutant Reveals a Mechanism for Metabolic Adaptation to Nitrogen-Limited Conditions
T Nakajima, K Yoshikawa, Y Toya… - Plant and Cell …, 2017 - academic.oup.com
Metabolic flux redirection during nitrogen-limited growth was investigated in the
Synechocystis sp. PCC 6803 glucose-tolerant (GT) strain under photoautotrophic conditions …
Synechocystis sp. PCC 6803 glucose-tolerant (GT) strain under photoautotrophic conditions …