Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that
has evolved into an indispensable approach for analyzing the function of genes. It …

CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …

CRISPR/Cas-based genome editing technologies have the potential to fast-track large-scale
crop breeding programs. However, the rigid cell wall limits the delivery of CRISPR/Cas …

Plant biotechnology is rife with new advances in transformation and genome engineering
techniques. A common requirement for delivery and coordinated expression in plant cells …

A persistent issue in the agricultural sector worldwide is the intensive damage caused to
crops by the geminivirus family of viruses. The diverse types of viruses, rapid virus evolution …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are …

The tribe Triticeae includes important agricultural crops, such as bread wheat, durum wheat,
barley, rye, and triticale. Research in the field of reverse genetics and genetic engineering of …

Infectious clone technology is universally applied for biological characterization and
engineering of viruses. This protocol describes procedures that implement synthetic biology …

Genome editing holds great promise for the molecular breeding of plants, yet its application
is hindered by the shortage of simple and effective means of delivering genome editing …