Clinical Diagnostics of Bacterial Infections and Their Resistance to Antibiotics—Current State and Whole Genome Sequencing Implementation Perspectives

E Avershina, A Khezri, R Ahmad - Antibiotics, 2023 - mdpi.com
Antimicrobial resistance (AMR), defined as the ability of microorganisms to withstand
antimicrobial treatment, is responsible for millions of deaths annually. The rapid spread of …

Highly sensitive quantitative phase microscopy and deep learning aided with whole genome sequencing for rapid detection of infection and antimicrobial resistance

A Ahmad, R Hettiarachchi, A Khezri… - Frontiers in …, 2023 - frontiersin.org
Current state-of-the-art infection and antimicrobial resistance (AMR) diagnostics are based
on culture-based methods with a detection time of 48–96 h. Therefore, it is essential to …

Short turnaround time of seven to nine hours from sample collection until informed decision for sepsis treatment using nanopore sequencing

J Ali, W Johansen, R Ahmad - Scientific Reports, 2024 - nature.com
Bloodstream infections (BSIs) and sepsis are major health problems, annually claiming
millions of lives. Traditional blood culture techniques, employed to identify sepsis-causing …

A practical guide to diagnosing bovine mastitis: a review

M Ramuada, TL Tyasi, L Gumede… - Frontiers in Animal …, 2024 - frontiersin.org
Mastitis is one of the major diseases affecting the viability of dairy farming due to direct and
indirect losses associated with low milk yield and poor milk quality. This review aims to …

Bacterial enrichment prior to third-generation metagenomic sequencing improves detection of BRD pathogens and genetic determinants of antimicrobial resistance in …

EK Herman, SR Lacoste, CN Freeman… - Frontiers in …, 2024 - frontiersin.org
Introduction Bovine respiratory disease (BRD) is one of the most important animal health
problems in the beef industry. While bacterial culture and antimicrobial susceptibility testing …

Whole genome sequencing in the palm of your hand: how to implement a MinION Galaxy-based workflow in a food safety laboratory for rapid Salmonella spp …

A Lamas, A Garrido-Maestu, A Prieto… - Frontiers in …, 2023 - frontiersin.org
Introduction Whole Genome Sequencing (WGS) implementation in food safety laboratories
is a significant advancement in food pathogen control and outbreak tracking. However, the …

Isolation and Identification of Microorganisms and Antibiotic Resistance Microorganisms from Beehives Located in Palm, Corn and Longan Plantations, Thailand

S Dokuta, S Yadoung, P Jeeno, S Hongjaisee… - Microorganisms, 2023 - mdpi.com
This study aims to determine the prevalence of microorganisms and antibiotic-resistant
microorganisms in beehives located on different plantations in Thailand. Seventeen swabs …

Non-Targeted RNA Sequencing: Towards the Development of Universal Clinical Diagnosis Methods for Human and Veterinary Infectious Diseases

S Spatz, CL Afonso - Veterinary Sciences, 2024 - mdpi.com
Simple Summary With advances in NGS technology, the high ratio of throughput/cost per
base has allowed research laboratories to develop diagnostic protocols that can detect …

Real-time Taxonomic Characterization of Long-read Mixed-species Sequencing Samples in Sorted Motif Distance Space: Voyager

S Branders, MG Grabherr, R Ahmad - bioRxiv, 2024 - biorxiv.org
Recent advances in long-read sequencing technology enable its use in potentially life-
saving applications for rapid clinical diagnostics and epidemiological monitoring. To take …

From Lab to Market: A Study on Commercializing A Novel Genomic Solution for Mastitis Diagnosis and Antimicrobial Resistance Control.

S Dhar - 2024 - brage.inn.no
Bovine mastitis is a major issue in dairy herds because it has a considerable financial
impact. Therefore, it is important to quickly and accurately diagnose it in order to prevent …