Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level
Protein-protein and protein-RNA interactions are essential for cell function and survival.
These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular …
These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular …
[HTML][HTML] Current Opinion in Chemical Biology: Issue: Chemical Biology of Protein Aggregation and phase separation
Protein-protein and protein-RNA interactions are essential for cell function and survival.
These interactions facilitate the formation of ribonucleoprotein (RNP) complexes and …
These interactions facilitate the formation of ribonucleoprotein (RNP) complexes and …
The roles of FUS-RNA binding domain and low complexity domain in RNA-dependent phase separation
Fused in sarcoma (FUS) is an archetypal phase separating protein asymmetrically divided
into a low complexity domain (LCD) and an RNA binding domain (RBD). Here, we explore …
into a low complexity domain (LCD) and an RNA binding domain (RBD). Here, we explore …
Single-cell RNA sequencing across isogenic FUS and TARDBP ALS lines reveals a shared early mitochondrial dysfunction unique to motor neurons
C Schweingruber, J Nijssen, J Mechtersheimer… - bioRxiv, 2023 - biorxiv.org
Mutations in the RNA/DNA-binding proteins FUS and TDP-43 cause the fatal disease
amyotrophic lateral sclerosis (ALS). The precise mechanisms behind the selective motor …
amyotrophic lateral sclerosis (ALS). The precise mechanisms behind the selective motor …
[HTML][HTML] Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
Mutations in intrinsically disordered proteins drive the irreversible formation of pathological
aggregates, a hallmark of neurodegenerative diseases. Here, we present a protocol to pull …
aggregates, a hallmark of neurodegenerative diseases. Here, we present a protocol to pull …
ALS/FTLD-Linked Mutant FUS Oligomerization in Cells
NA Djaja - 2024 - jscholarship.library.jhu.edu
Fused in sarcoma (FUS) is a nuclear RNA-binding protein (RBP) implicated in amyotrophic
lateral sclerosis (ALS) and frontotemporal lobar dementia (FTLD). FUS plays essential roles …
lateral sclerosis (ALS) and frontotemporal lobar dementia (FTLD). FUS plays essential roles …