Single-cell microfluidic impedance cytometry: From raw signals to cell phenotypes using data analytics

C Honrado, P Bisegna, NS Swami, F Caselli - Lab on a Chip, 2021 - pubs.rsc.org
The biophysical analysis of single-cells by microfluidic impedance cytometry is emerging as
a label-free and high-throughput means to stratify the heterogeneity of cellular systems …

Microfluidic impedance cytometry for single-cell sensing: Review on electrode configurations

S Zhu, X Zhang, Z Zhou, Y Han, N Xiang, Z Ni - Talanta, 2021 - Elsevier
Single-cell analysis has gained considerable attention for disease diagnosis, drug
screening, and differentiation monitoring. Compared to the well-established flow cytometry …

Microscopic impedance cytometry for quantifying single cell shape

T Tang, X Liu, R Kiya, Y Shen, Y Yuan, T Zhang… - Biosensors and …, 2021 - Elsevier
In this work, we investigated the ability of impedance flow cytometry to measure the shape of
single cells/particles. We found that the impedance pulses triggered by micro-objects that …

Coplanar electrode microfluidic chip enabling accurate sheathless impedance cytometry

A De Ninno, V Errico, FR Bertani, L Businaro… - Lab on a Chip, 2017 - pubs.rsc.org
Microfluidic impedance cytometry offers a simple non-invasive method for single-cell
analysis. Coplanar electrode chips are especially attractive due to ease of fabrication …

Chemical analysis of single cells

Y Lin, R Trouillon, G Safina, AG Ewing - Analytical Chemistry, 2011 - ACS Publications
In this Review, we attempt to give an overview of methods developed for chemical analysis
of single cells. This is an important topic for several reasons. Many biological systems have …

High accuracy particle analysis using sheathless microfluidic impedance cytometry

D Spencer, F Caselli, P Bisegna, H Morgan - Lab on a Chip, 2016 - pubs.rsc.org
This paper describes a new design of microfluidic impedance cytometer enabling accurate
characterization of particles without the need for focusing. The approach uses multiple pairs …

[HTML][HTML] A novel wiring scheme for standard chips enabling high-accuracy impedance cytometry

F Caselli, A De Ninno, R Reale, L Businaro… - Sensors and Actuators B …, 2018 - Elsevier
While there is a great interest in microfluidic impedance cytometry as a label-free approach
for single-particle analysis, the accuracy of the technique is challenged by the positional …

On the compatibility of single-cell microcarriers (nanovials) with microfluidic impedance cytometry

C Brandi, A De Ninno, F Ruggiero, E Limiti… - Lab on a Chip, 2024 - pubs.rsc.org
We investigate for the first time the compatibility of nanovials with microfluidic impedance
cytometry (MIC). Nanovials are suspendable crescent-shaped single-cell microcarriers that …

An impedance-based flow microcytometer for single cell morphology discrimination

M Shaker, L Colella, F Caselli, P Bisegna, P Renaud - Lab on a Chip, 2014 - pubs.rsc.org
Cell shape is a fundamental biological feature, providing specific information about
physiological or pathological cellular conditions. Most of the state-of-the-art microfluidic …

High-throughput electrical position detection of single flowing particles/cells with non-spherical shape

R Reale, A De Ninno, L Businaro, P Bisegna, F Caselli - Lab on a Chip, 2019 - pubs.rsc.org
We present an innovative impedance cytometer for the measurement of the cross-sectional
position of single particles or cells flowing in a microchannel. As predicted by numerical …