Understanding the rules of life is one of the most important scientific endeavours and has
revolutionised both biology and biotechnology. Remarkable advances in observation …

We review the standard model for de novo computational design of enzymes, which
primarily focuses on the development of an active-site geometry, composed of protein …

A NEW YORK TIMES BESTSELLER “Brilliant and enthralling.”​—The Wall Street Journal A
paradigm-shifting book from an acclaimed Harvard Medical School scientist and one of …

The enormous rate accelerations observed for many enzyme catalysts are due to strong
stabilizing interactions between the protein and reaction transition state. The defining …

Many enzymes that show a large specificity in binding the enzymatic transition state with a
higher affinity than the substrate utilize substrate binding energy to drive protein …

Using high-throughput microfluidic enzyme kinetics (HT-MEK), we measured over 9,000
inhibition curves detailing impacts of 1,004 single-site mutations throughout the alkaline …

The task of adapting enzymes for specific applications is often hampered by our incomplete
ability to tune and tailor catalytic functions, particularly when seeking increased activity …

How enzymes achieve their enormous rate enhancements remains a central question in
biology, and our understanding to date has impacted drug development, influenced enzyme …

Enzymes host active sites inside protein macromolecules, which have diverse, often
incredibly complex, and atom-expensive structures. It is an outstanding question what the …

Conventional understanding of how enzymes function strongly emphasizes the role of
structure. However, increasing evidence clearly indicates that enzymes do not remain fixed …