Evolution of exon-intron structure of eukaryotic genes has been a matter of long-standing,
intensive debate. The introns-early concept, later rebranded 'introns first'held that protein …

The splicing of precursors to mRNAs occurs in two steps, both involving single
transesterification reactions (Fig. 1). The first step generates a 2-5 bond at the branch site …

In nuclear pre-messenger RNA splicing, introns are excised by the spliceosome, a dynamic
machine composed of both proteins and small nuclear RNAs (snRNAs). Over thirty years …

Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and
extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo …

The spliceosome catalyses the excision of introns from pre-mRNA in two steps, branching
and exon ligation, and is assembled from five small nuclear ribonucleoprotein particles …

The spliceosome excises introns from pre-mRNAs in two sequential transesterifications—
branching and exon ligation—catalysed at a single catalytic metal site in U6 small nuclear …

Intervening sequences are removed from nuclear pre-mRNAs in a well-defined multi-step
pathway. Small nuclear ribonucleoprotein particles (snRNPs) and numerous protein factors …

Pre-messenger RNA (pre-mRNA) splicing is a central step in gene expression. Lying
between transcription and protein synthesis, pre-mRNA splicing removes sequences …

Alternative splicing enhances transcriptome diversity in all eukaryotes and plays a role in
plant tissue identity and stress adaptation. To catalog new maize (Zea mays) transcripts and …

Pre-mRNA splicing requires dramatic RNA rearrangements hypothesized to be catalyzed by
ATP-dependent RNA unwindases of the DExD/H box family. In a rearrangement critical for …