Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Microfluidics: innovations in materials and their fabrication and functionalization

JB Nielsen, RL Hanson, HM Almughamsi… - Analytical …, 2019 - ACS Publications
Microfluidics is a growing field of study because these devices offer novel and versatile
approaches for addressing a range of scientific problems. Microfluidics have been used in …

Structural and thermoanalytical characterization of 3D porous PDMS foam materials: the effect of impurities derived from a sugar templating process

J González-Rivera, R Iglio, G Barillaro, C Duce… - Polymers, 2018 - mdpi.com
Polydimethylsiloxane (PDMS) polymers are extensively used in a wide range of research
and industrial fields, due to their highly versatile chemical, physical, and biological …

Chemical Imaging of Mass Transport Near the No-Slip Interface of a Microfluidic Device using Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy

GT Flaman, ND Boyle, C Vermelle, TA Morhart… - Analytical …, 2023 - ACS Publications
Mass transport in geometrically confined environments is fundamental to microfluidic
applications. Measuring the distribution of chemical species on flow requires the use of …

Microfabrication process development for a polymer-based lab-on-chip concept applied in attenuated total reflection fourier transform infrared spectroelectrochemistry

N Atkinson, TA Morhart, G Wells, GT Flaman, E Petro… - Sensors, 2023 - mdpi.com
Micro electro-mechanical systems (MEMS) combining sensing and microfluidics
functionalities, as are common in Lab-on-Chip (LoC) devices, are increasingly based on …

Measuring proteins in H 2 O with 2D-IR spectroscopy

S Hume, G Hithell, GM Greetham, PM Donaldson… - Chemical …, 2019 - pubs.rsc.org
The amide I infrared band of proteins is highly sensitive to secondary structure, but studies
under physiological conditions are prevented by strong, overlapping water absorptions …

Continuous-flow enzymatic synthesis of chiral lactones in a three-dimensional microfluidic reactor

X Deng, M Fan, M Wu, X Zhang, Y Cheng, J Xia… - Chinese Chemical …, 2024 - Elsevier
A new continuous-flow process for the enzymatic synthesis of optically pure γ-lactones,
which are used as flavors and fragrances in the food and cosmetic industries, was …

The present and future role of microfluidics for protein and peptide-based therapeutics and diagnostics

E Weaver, S Uddin, DK Cole, A Hooker, DA Lamprou - Applied Sciences, 2021 - mdpi.com
The implementation of peptide-based molecules within the medical field has vast potential,
owing to their unique nature and predictable physicochemical profiles. However, peptide …

[HTML][HTML] Shining new light into soil systems: spectroscopy in microfluidic soil chips reveals microbial biogeochemistry

M Pucetaite, P Ohlsson, P Persson… - Soil Biology and …, 2021 - Elsevier
Microfluidic soil chips render optical access to the naturally opaque soil systems and enable
direct investigation of microbial growth and interactions in micro-structurally and chemically …

High-throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform

S Neun, L van Vliet, F Hollfelder, F Gielen - Analytical Chemistry, 2022 - ACS Publications
Microfluidic water-in-oil emulsion droplets are becoming a mainstay of experimental biology,
where they replace the classical test tube. In most applications, such as ultrahigh-throughput …