Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Cryo‐electron microscopy (cryo‐EM) is increasingly becoming a mainstream technology for
studying the architecture of cells, viruses and protein assemblies at molecular resolution …

Correlative electron and fluorescence microscopy has the potential to elucidate the
ultrastructural details of dynamic and rare cellular events, but has been limited by low …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Sample fixation by vitrification is critical for the optimal structural preservation of
biomolecules and subsequent high-resolution imaging by cryo-correlative light and electron …

Cryo-electron tomography (cryo-ET) is a revolutionary technique for resolving the structure
of subcellular organelles and macromolecular complexes in their cellular context. However …