Pichia pastoris is one of the most widely used host for the production of recombinant
proteins. Expression systems that rely mostly on promoters from genes encoding alcohol …

The fed-batch cultivation is in many ways a benchmark for fermentation processes, and it
has been an attractive choice for the biotechnological production of various products in the …

High-cell density cultures (HCDC), prone to metabolic overflow, are typically operated in fed-
batch mode to maximize productivity. We developed a simulation-based procedure for …

Real‐time estimation of physiological properties of the cell during recombinant protein
production would ensure enhanced process monitoring. In this study, we explored the …

Dissolved carbon dioxide (dCO2) is a well‐known critical parameter in bioprocesses due to
its significant impact on cell metabolism and on product quality attributes. Processes run at …

BACKGROUND In recent decades, artificial neural network (ANN) has been shown to be a
robust and promising tool in monitoring and controlling bioprocess systems. In a previous …

Mass transfer is known to play a critical role in bioprocess performance and henceforth
monitoring dissolved O2 (DO) and dissolved CO2 (dCO2) is of paramount importance. At …

Neural networks can provide highly accurate and robust solutions for complex non-linear
kinetic like bioprocess reactions. It is applied as a soft sensor in the biotechnology industry …

In this work, a new adaptive controller is designed for substrate control of a fed-batch
bioreactor in the presence of input saturation and unknown varying control gain with …

In this work, substrate control of a biological process with unknown varying control gain,
input saturation, and uncertain reaction rate is addressed. A novel adaptive controller is …