In the last years, tremendous progress has been made in the development of CRISPR/Cas-
mediated genome editing tools. A number of natural CRISPR/Cas nuclease variants have …

New tools and advanced technologies have played key roles in facilitating basic research in
plant pathology and practical approaches for disease management and crop health …

CRISPR/Cas genome-editing tools provide unprecedented opportunities for basic plant
biology research and crop breeding. However, the lack of robust delivery methods has …

Plant genetic transformation is a crucial step for applying biotechnology such as genome
editing to basic and applied plant science research. Its success primarily relies on the …

Since its inception in 2012, CRISPR-Cas technologies have taken the life science
community by storm. Maize genetics research is no exception. Investigators around the …

Plant genome editing, particularly CRISPR-Cas biotechnologies, has rapidly evolved and
drawn enormous attention all around the world in the last decade. The cutting-edge …

Viral vectors hold enormous potential for genome editing in plants as transient delivery
vehicles of CRISPR-Cas components. Here, we describe a protocol to assemble plant viral …

The requirement of in vitro tissue culture for the delivery of gene editing reagents limits the
application of gene editing to commercially relevant varieties of many crop species. To …

The ever-increasing demand for vegetable oil requirement has necessitated increasing the
production of oilseeds crops. Productivity is compromised in these crops due to biotic and …

Efficient transformation systems are highly desirable for plant genetic research and
biotechnology product development efforts. Tissue culture‐free transformation (TCFT) and …