[PDF][PDF] Fluorescent toys 'n'tools lighting the way in fungal research

W Van Genechten, P Van Dijck… - FEMS Microbiology …, 2021 - academic.oup.com
Although largely overlooked compared to bacterial infections, fungal infections pose a
significant threat to the health of humans and other organisms. Many pathogenic fungi …

Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa

Z Wang, BM Bartholomai, JJ Loros… - Fungal Genetics and …, 2023 - Elsevier
Fungal cells are quite unique among life in their organization and structure, and yet
implementation of many tools recently developed for fluorescence imaging in animal …

A multi-colour fluorogenic tag and its application in Candida albicans

J Devos, P Van Dijck, W Van Genechten - Microbiology, 2024 - microbiologyresearch.org
Fluorescent proteins (FPs) have always been a crucial part of molecular research in life
sciences, including the research into the human fungal pathogen Candida albicans, but …

Choosing the Right Fluorescent Probe

MJ Sarmento, F Fernandes - Fluorescence Spectroscopy and Microscopy …, 2023 - Springer
Fluorescence microscopy and spectroscopy are by now used routinely in any laboratory
working on the field of basic and applied biological sciences. A wide and expanding library …

蓝色和黄色荧光蛋白在灵芝中的表达及黄色荧光蛋白在诱导型启动子Pcbh2 活性分析中的应用.

黄雄敏, 徐艳, 徐军伟 - Mycosystema, 2024 - search.ebscohost.com
本研究中通过密码子优化和内含子添加分别构建了蓝色荧光蛋白基因(bfp) 表达载体PJW-EXP-
intron-opbfp 和黄色荧光蛋白基因(yfp) 表达载体PJW-EXP-intron-opyfp, 使用灵芝Ganoderma …

Photochromic fluorophores enable imaging of lowly expressed proteins in the autofluorescent fungus Candida albicans

W Van Genechten, L Demuyser, S Duwé… - Msphere, 2021 - Am Soc Microbiol
Fluorescence microscopy is a standard research tool in many fields, although collecting
reliable images can be difficult in systems characterized by low expression levels and/or …

Abundance-Biased Codon Diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors

L Ravotto, J Dernic, A Eleftheriou, M Rauch, P Imseng… - 2024 - researchsquare.com
The delivery of genetically encoded fluorescent sensors via adeno-associated viral vectors
(AAVs) enables the quantification of biological analytes with high spatiotemporal resolution …

Differential sensing by the C. albicans Gpr1 receptor results in morphogenesis, β-glucan masking and survival in macrophages

W Van Genechten, S Wijnants, J Vreys, P Van Dijck - bioRxiv, 2022 - biorxiv.org
The human fungal pathogen, Candida albicans, is very proficient at several classical
virulence factors such as morphogenesis, adhesion, biofilm formation and immune evasion …

Structural Determinants of Blue to Red Fluorescent Protein Conversion

SS Lambert, R Larsen, NE Wheatley… - Transactions of the …, 2022 - BioOne
The color, brightness, and stability of a fluorescent protein are dictated by its amino acid
sequence. Attempts to alter the physical properties of fluorescent proteins have focused on …

The role of carboxylic acids in pathogenicity of Candida albicans

F Ghasemi - 2022 - search.proquest.com
In recent decades, fungal pathogens have become a global health problem. Candida
albicans, a common opportunistic commensal microorganism, is the most frequent cause of …