Point-of-care isothermal nucleic acid amplification tests: progress and bottlenecks for extraction-free sample collection and preparation

AF Wilkinson, MJ Barra, EN Novak… - Expert Review of …, 2024 - Taylor & Francis
Introduction Suitable sample collection and preparation methods are essential to enable
nucleic acid amplification testing at the point of care (POC). Strategies that allow direct …

Method versatility in RNA extraction-free PCR detection of SARS-CoV-2 in saliva samples

OM Allicock, D Yolda-Carr, R Earnest, MI Breban… - Progress in Biophysics …, 2023 - Elsevier
Early in the pandemic, a simple, open-source, RNA extraction-free RT-qPCR protocol for
SARS-CoV-2 detection in saliva was developed and made widely available. This simplified …

Persistence of pneumococcal carriage among older adults in the community despite COVID-19 mitigation measures

AL Wyllie, S Mbodj, DA Thammavongsa… - Microbiology …, 2023 - Am Soc Microbiol
Reported rates of invasive pneumococcal disease were markedly lower than normal during
the 2020/2021 winter in the Northern Hemisphere, the first year after the start of the COVID …

Quantitative serology for SARS-CoV-2 using self-collected saliva and finger-stick blood

C Campbell, N Padmanabhan, D Romero, J Joe… - Scientific Reports, 2022 - nature.com
Convenient and widespread serology testing may alter the trajectory of the COVID-19
pandemic. This study seeks to leverage high-throughput, multiplexed serologic assays …

A community study of SARS-CoV-2 detection by RT-PCR in saliva: a reliable and effective method

F Fronza, N Groff, A Martinelli, BZ Passerini, N Rensi… - Viruses, 2022 - mdpi.com
Efficient, wide-scale testing for SARS-CoV-2 is crucial for monitoring the incidence of the
infection in the community. The gold standard for COVID-19 diagnosis is the molecular …

Exploring the potential of a saliva-based, RNA-extraction-free PCR test for the multiplexed detection of key respiratory pathogens

OM Allicock, TY Lin, KT Fajardo, D Yolda-Carr… - medRxiv, 2023 - medrxiv.org
Introduction Efforts to diagnose and monitor transmissible respiratory infections can be
impaired by invasive or resource-intensive sample collection. Having extensively …

SHEAR saliva collection device augments sample properties for improved analytical performance

SW Song, R Gupta, N Jothilingam… - Bioengineering & …, 2023 - Wiley Online Library
Despite being a convenient clinical substrate for biomonitoring, saliva's widespread
utilization has not yet been realized. The non‐Newtonian, heterogenous, and highly viscous …

Scalable solutions for global health: the SalivaDirect model

AL Wyllie, B Choate, L Burke, Y Ali - Frontiers in Cellular and Infection …, 2024 - frontiersin.org
The COVID-19 pandemic caught the world unprepared. Large-scale testing efforts were
urgently needed, and diagnostic strategies had to rapidly evolve in response to …

Saliva as an alternative sample type for detection of pneumococcal carriage in young children

AL Wyllie, NY Rots, AJ Wijmenga-Monsuur… - …, 2023 - microbiologyresearch.org
For children, the gold standard for the detection of pneumococcal carriage is conventional
culture of a nasopharyngeal swab. Saliva, however, has a history as one of the most …

Diagnostic performance, stability, and acceptability of self-collected saliva without additives for SARS-CoV-2 molecular diagnosis

C Marín-Echeverri, L Pérez-Zapata… - European Journal of …, 2024 - Springer
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV‐2) caused the
coronavirus disease 2019 (COVID-19), leading to a global pandemic. The molecular …