Modern trends in plant genome editing: an inclusive review of the CRISPR/Cas9 toolbox
Increasing agricultural productivity via modern breeding strategies is of prime interest to
attain global food security. An array of biotic and abiotic stressors affect productivity as well …
attain global food security. An array of biotic and abiotic stressors affect productivity as well …
INDEL detection, the 'Achilles heel'of precise genome editing: a survey of methods for accurate profiling of gene editing induced indels
EP Bennett, BL Petersen, IE Johansen… - Nucleic acids …, 2020 - academic.oup.com
Advances in genome editing technologies have enabled manipulation of genomes at the
single base level. These technologies are based on programmable nucleases (PNs) that …
single base level. These technologies are based on programmable nucleases (PNs) that …
A survey of validation strategies for CRISPR-Cas9 editing
MF Sentmanat, ST Peters, CP Florian, JP Connelly… - Scientific reports, 2018 - nature.com
Abstract The T7 endonuclease 1 (T7E1) mismatch detection assay is a widely used method
for evaluating the activity of site-specific nucleases, such as the clustered regularly …
for evaluating the activity of site-specific nucleases, such as the clustered regularly …
Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems
Q Liu, C Wang, X Jiao, H Zhang, L Song, Y Li… - Science China Life …, 2019 - Springer
The CRISPR/Cas system has been extensively applied to make precise genetic
modifications in various organisms. Despite its importance and widespread use, large-scale …
modifications in various organisms. Despite its importance and widespread use, large-scale …
Gene-edited human stem cell–derived β cells from a patient with monogenic diabetes reverse preexisting diabetes in mice
KG Maxwell, P Augsornworawat… - Science translational …, 2020 - science.org
Differentiation of insulin-producing pancreatic β cells from induced pluripotent stem cells
(iPSCs) derived from patients with diabetes promises to provide autologous cells for …
(iPSCs) derived from patients with diabetes promises to provide autologous cells for …
Genome-wide targeted mutagenesis in rice using the CRISPR/Cas9 system
Since the completion of the rice (Oryza sativa) genomesequencing project, a major goal of
rice research has been the functional characterization of all annotated genetic loci in various …
rice research has been the functional characterization of all annotated genetic loci in various …
Microhomologies are prevalent at Cas9-induced larger deletions
DDG Owens, A Caulder, V Frontera… - Nucleic acids …, 2019 - academic.oup.com
The CRISPR system is widely used in genome editing for biomedical research. Here, using
either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize …
either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize …
[HTML][HTML] CRISPR-Cas9 library screening approach for anti-cancer drug discovery: overview and perspectives
CRISPR-Cas9 is a Nobel Prize-winning robust gene-editing tool developed in the last
decade. This technique enables a stable genetic engineering method with high precision on …
decade. This technique enables a stable genetic engineering method with high precision on …
Next-generation sequencing and genome editing in plant virology
A Hadidi, R Flores, T Candresse, M Barba - Frontiers in microbiology, 2016 - frontiersin.org
Next-generation sequencing (NGS) has been applied to plant virology since 2009. NGS
provides highly efficient, rapid, low cost DNA, or RNA high-throughput sequencing of the …
provides highly efficient, rapid, low cost DNA, or RNA high-throughput sequencing of the …
Editing the genome of hiPSC with CRISPR/Cas9: disease models
AR Bassett - Mammalian Genome, 2017 - Springer
The advent of human-induced pluripotent stem cell (hiPSC) technology has provided a
unique opportunity to establish cellular models of disease from individual patients, and to …
unique opportunity to establish cellular models of disease from individual patients, and to …