Glycans are involved in numerous biological recognition events. Being secondary gene
products, their labeling by genetic methods–comparable to GFP labeling of proteins–is not …

The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Despite advances in genetic and proteomic techniques, a complete portrait of the proteome
and its complement of dynamic interactions and modifications remains a lofty, and as of yet …

The addition of O-linked-β-DN-acetylglucosamine (O-GlcNAc) onto serine and threonine
residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational …

Computation has guided the design of conformationally-strained dioxolane-fused trans-
cyclooctene (d-TCO) derivatives that display excellent reactivity in the tetrazine ligation. A …

The surging development of bioorthogonal chemistry has profoundly transformed chemical
biology over the last two decades. Involving chemical partners that specifically react together …

Conspectus Chemical tools are transforming our understanding of biomolecules and living
systems. Included in this group are bioorthogonal reagents–functional groups that are inert …

The Diels–Alder reactions of seven 1, 2, 4, 5-tetrazines with unstrained and strained alkenes
and alkynes were studied with quantum mechanical calculations (M06-2X density functional …

A new class of bioorthogonal reagents, 1, 2, 4-triazines, is described. These scaffolds are
stable in biological media and capable of robust reactivity with trans-cyclooctene (TCO). The …

Recent developments in fluorescence microscopy call for novel small‐molecule‐based
labels with multiple functionalities to satisfy different experimental requirements. A current …