cDNA microarray reveals the alterations of cytoskeleton-related genes in osteoblast under high magneto-gravitational environment

A Qian, S Di, X Gao, W Zhang, Z Tian, J Li… - Acta Biochim …, 2009 - academic.oup.com
The diamagnetic levitation as a novel ground-based model for simulating a reduced gravity
environment has been widely applied in many fields. In this study, a special designed …

High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

AR Qian, PF Yang, LF Hu, W Zhang, SM Di… - Advances in space …, 2010 - Elsevier
The effects of a high magnetic gradient environment (HMGE) on the cytoskeletal architecture
and genes associated with the cytoskeleton in osteoblasts (MC3T3-E1 and MG-63 cells) …

Physical forces modulate oxidative status and stress defense meditated metabolic adaptation of yeast colonies: Spaceflight and microgravity simulations

TG Hammond, PL Allen, MA Gunter, J Chiang… - … Science and Technology, 2018 - Springer
Baker's yeast (Saccharomyces cerevisiae) has broad genetic homology to human cells.
Although typically grown as 1-2mm diameter colonies under certain conditions yeast can …

Enhancement of recombinant β‐D‐glucuronidase production under low‐shear modeled microgravity in Pichia pastoris

F Qi, I Kaleem, B Lv, X Guo, C Li - Journal of Chemical …, 2011 - Wiley Online Library
BACKGROUND: The effects of low‐shear modeled microgravity (LSMMG) on the production
of recombinant proteins in a eukaryotic expression host have been investigated. The gene …

Regulatory snapshots: integrative mining of regulatory modules from expression time series and regulatory networks

JP Gonçalves, RS Aires, AP Francisco, SC Madeira - PloS one, 2012 - journals.plos.org
Explaining regulatory mechanisms is crucial to understand complex cellular responses
leading to system perturbations. Some strategies reverse engineer regulatory interactions …

Novel Sfp1 Transcriptional Regulation of Saccharomyces cerevisiae Gene Expression Changes During Spaceflight

CB Coleman, PL Allen, M Rupert, C Goulart, A Hoehn… - Astrobiology, 2008 - liebertpub.com
This study identifies transcriptional regulation of stress response element (STRE) genes in
space in the model eukaryotic organism, Saccharomyces cerevisiae. To determine …

Effects of Low-Shear Modeled Microgravity on the Characterization of Recombinant β-D-Glucuronidase Expressed in Pichia pastoris

F Qi, DZ Dai, Y Liu, I Kaleem, C Li - Applied biochemistry and …, 2011 - Springer
In this study, we used a high-aspect-ratio vessel (HARV), which could model environment of
microgravity on ground to investigate for the first time the effects of low-shear modeled …

Flow cytometry of yeasts and other fungi

T Overton - Flow cytometry in Microbiology: Technology …, 2015 - research.birmingham.ac.uk
Flow cytometry is used extensively to investigate many aspects of yeast physiology and cell
biology. This chapter describes the most common applications of flow cytometry and …

On the nature and shape of tubulin trails: Implications on microtubule self-organization

N Glade - Acta biotheoretica, 2012 - Springer
Microtubules, major elements of the cell skeleton are, most of the time, well organized in
vivo, but they can also show self-organizing behaviors in time and/or space in purified …

Establishing a low redox potential in giant yeast colonies: effects of media and rotation

HH Birdsall, PL Allen, JS Hammond… - … and Space Research, 2016 - sciendo.com
Giant yeast colonies develop a low redox potential, which mimics the electrophilic milieu of
both the mitochondrial drug metabolizing compartment and the hypoxic core of many …