Rapid PCR powered by microfluidics: A quick review under the background of COVID-19 pandemic

X Dong, L Liu, Y Tu, J Zhang, G Miao, L Zhang… - TrAC Trends in …, 2021 - Elsevier
PCR has been widely used in different fields including molecular biology, pathogen
detection, medical diagnosis, food detection and etc. However, the difficulty of promoting …

[HTML][HTML] CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives

T Huang, R Zhang, J Li - Journal of Advanced Research, 2023 - Elsevier
Background Early detection of pathogen-associated diseases are critical for effective
treatment. Rapid, specific, sensitive, and cost-effective diagnostic technologies continue to …

DropCRISPR: A LAMP-Cas12a based digital method for ultrasensitive detection of nucleic acid

H Wu, X Cao, Y Meng, D Richards, J Wu, Z Ye… - Biosensors and …, 2022 - Elsevier
Since their discovery, CRISPR/Cas systems have been extensively exploited in nucleic acid
biosensing. However, the vast majority of contemporary platforms offer only qualitative …

A reversible valve-assisted chip coupling with integrated sample treatment and CRISPR/Cas12a for visual detection of Vibrio parahaemolyticus

H Wu, Y Chen, Q Yang, C Peng, X Wang… - Biosensors and …, 2021 - Elsevier
Vibrio parahaemolyticus (V. parahaemolyticus) is regarded as a major cause of seafood-
associated illnesses, which has aroused widespread public concern. Here, a rapid and …

Quantitative or digital PCR? A comparative analysis for choosing the optimal one for biosensing applications

H Zhang, L Cao, J Brodsky, I Gablech, F Xu, Z Li… - TrAC Trends in …, 2024 - Elsevier
This paper offers an exhaustive comparison of quantitative PCR (qPCR) and digital PCR
(dPCR) technologies, focusing on their critical roles in molecular diagnostics, ranging from …

A highly sensitive, real-time centrifugal microfluidic chip for multiplexed detection based on isothermal amplification

X Dong, Z Tang, X Jiang, Q Fu, D Xu, L Zhang, X Qiu - Talanta, 2024 - Elsevier
A real-time centrifugal microfluidic chip with a companion analyzer was developed for highly
sensitive, multiplexed nucleic acid detection based on RPA (recombinase polymerase …

Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

Y Wang, C Wang, Z Zhou, J Si, S Li, Y Zeng, Y Deng… - Biosensors, 2023 - mdpi.com
Pathogenic pathogens invade the human body through various pathways, causing damage
to host cells, tissues, and their functions, ultimately leading to the development of diseases …

Precise determination of reaction conditions for accurate quantification in digital PCR by real-time fluorescence monitoring within microwells

Q Xu, J Li, Z Zhang, Q Yang, W Zhang, J Yao… - Biosensors and …, 2024 - Elsevier
Real-time digital polymerase chain reaction (qdPCR) provides enhanced precision in the
field of molecular diagnostics by integrating absolute quantification with process information …

Rapid Nucleic Acid Diagnostic Technology for Pandemic Diseases

Y Lei, D Xu - Molecules, 2024 - mdpi.com
The recent global pandemic of coronavirus disease 2019 (COVID-19) has enormously
promoted the development of diagnostic technology. To control the spread of pandemic …

Knowledge about foodborne pathogens and PCR‐based microbial detection methods among Brazilian students and professionals: A survey

LMR Lemos, LD Silva, ACM Arisi - Journal of Food Safety, 2023 - Wiley Online Library
Polymerase chain reaction (PCR) is widely used in several research areas and routine
laboratory analyses, including foodborne pathogens detection. We aimed to investigate the …