Spatial omics and multiplexed imaging to explore cancer biology
Understanding intratumoral heterogeneity—the molecular variation among cells within a
tumor—promises to address outstanding questions in cancer biology and improve the …
tumor—promises to address outstanding questions in cancer biology and improve the …
Architectures of neuronal circuits
L Luo - Science, 2021 - science.org
BACKGROUND The human brain contains about 100 billion neurons, each of which makes
thousands of synaptic connections. Although individual neurons can themselves be …
thousands of synaptic connections. Although individual neurons can themselves be …
[HTML][HTML] Lineage tracing reveals the phylodynamics, plasticity, and paths of tumor evolution
Tumor evolution is driven by the progressive acquisition of genetic and epigenetic
alterations that enable uncontrolled growth and expansion to neighboring and distal tissues …
alterations that enable uncontrolled growth and expansion to neighboring and distal tissues …
Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain
MR Chuapoco, NC Flytzanis, N Goeden… - Nature …, 2023 - nature.com
Crossing the blood–brain barrier in primates is a major obstacle for gene delivery to the
brain. Adeno-associated viruses (AAVs) promise robust, non-invasive gene delivery from the …
brain. Adeno-associated viruses (AAVs) promise robust, non-invasive gene delivery from the …
High-definition spatial transcriptomics for in situ tissue profiling
Spatial and molecular characteristics determine tissue function, yet high-resolution methods
to capture both concurrently are lacking. Here, we developed high-definition spatial …
to capture both concurrently are lacking. Here, we developed high-definition spatial …
Tissue clearing
Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …
and has only recently found widespread use in the field of microscopy. This renaissance has …
Physiology of astroglia
A Verkhratsky, M Nedergaard - Physiological reviews, 2018 - journals.physiology.org
Astrocytes are neural cells of ectodermal, neuroepithelial origin that provide for homeostasis
and defense of the central nervous system (CNS). Astrocytes are highly heterogeneous in …
and defense of the central nervous system (CNS). Astrocytes are highly heterogeneous in …
Whole-body cellular mapping in mouse using standard IgG antibodies
Whole-body imaging techniques play a vital role in exploring the interplay of physiological
systems in maintaining health and driving disease. We introduce wildDISCO, a new …
systems in maintaining health and driving disease. We introduce wildDISCO, a new …
The emerging nature of astrocyte diversity
BS Khakh, B Deneen - Annual review of neuroscience, 2019 - annualreviews.org
Astrocytes are morphologically complex, ubiquitous cells that are viewed as a
homogeneous population tiling the entire central nervous system (CNS). However, this view …
homogeneous population tiling the entire central nervous system (CNS). However, this view …
Three-dimensional intact-tissue sequencing of single-cell transcriptional states
INTRODUCTION Single-cell RNA sequencing has demonstrated that both stable cell types
and transient cell states can be discovered and defined by transcriptomes. In situ …
and transient cell states can be discovered and defined by transcriptomes. In situ …