Association of alpha-crystallin with fiber cell plasma membrane of the eye lens accompanied by light scattering and cataract formation

R Timsina, L Mainali - Membranes, 2021 - mdpi.com
α-crystallin is a major protein found in the mammalian eye lens that works as a molecular
chaperone by preventing the aggregation of proteins and providing tolerance to stress in the …

Organization of lipids in fiber-cell plasma membranes of the eye lens

WK Subczynski, L Mainali, M Raguz… - Experimental eye …, 2017 - Elsevier
The plasma membrane together with the cytoskeleton forms the only supramolecular
structure of the matured fiber cell which accounts for mostly all fiber cell lipids. The purpose …

[HTML][HTML] Complexity of seemingly simple lipid nanodiscs

P Stepien, B Augustyn, C Poojari, W Galan… - … et Biophysica Acta (BBA …, 2020 - Elsevier
Lipid nanodiscs are macromolecular assemblies, where a scaffold protein is wrapped
around a nanosized disc of a lipid bilayer, thus protecting the hydrocarbon chains at the disc …

Changes in the properties and organization of human lens lipid membranes occurring with age

L Mainali, M Raguz, WJ O'Brien… - Current eye …, 2017 - Taylor & Francis
Purpose: This research was undertaken to document the changes in the organization and
properties of human lens lipid membranes that occur with age. Methods: Human lens lipid …

Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61–70-year-old human donors

L Mainali, M Raguz, WJ O'Brien… - European Biophysics …, 2015 - Springer
Human lens-lipid membranes prepared from the total lipids extracted from clear and
cataractous lens cortexes and nuclei of 61–70-year-old donors by use of a rapid solvent …

[HTML][HTML] Proteomic analysis of lipid raft-like detergent-resistant membranes of lens fiber cells

Z Wang, KL Schey - Investigative ophthalmology & visual …, 2015 - tvst.arvojournals.org
Purpose: Plasma membranes of lens fiber cells have high levels of long-chain saturated fatty
acids, cholesterol, and sphingolipids—key components of lipid rafts. Thus, lipid rafts are …

Multilamellar liposomes as a model for biological membranes: saturation recovery EPR spin-labeling studies

WK Subczynski, M Raguz, J Widomska - Membranes, 2022 - mdpi.com
EPR spin labeling has been used extensively to study lipids in model membranes to
understand their structures and dynamics in biological membranes. The lipid multilamellar …

Glycation of nail proteins: from basic biochemical findings to a representative marker for diabetic glycation-associated target organ damage

AS Kishabongo, P Katchunga, EH Van Aken… - PLoS …, 2015 - journals.plos.org
Background Although assessment of glycated nail proteins may be a useful marker for
monitoring of diabetes, their nature and formation are still poorly understood. Besides a …

Lipid domains in intact fiber-cell plasma membranes isolated from cortical and nuclear regions of human eye lenses of donors from different age groups

M Raguz, L Mainali, WJ O'Brien… - Experimental eye …, 2015 - Elsevier
The results reported here clearly document changes in the properties and the organization
of fiber-cell membrane lipids that occur with age, based on electron paramagnetic …

Characterization of the Distribution of Spin–Lattice Relaxation Rates of Lipid Spin Labels in Fiber Cell Plasma Membranes of Eye Lenses with a Stretched Exponential …

N Stein, L Mainali, JS Hyde, WK Subczynski - Applied magnetic …, 2019 - Springer
The stretched exponential function (SEF) was used to analyze and interpret saturation
recovery (SR) electron paramagnetic resonance (EPR) data obtained from spin-labeled …