Sequencing Strategy to Ensure Accurate Plasmid Assembly

SI Hernandez, CT Berezin, KM Miller… - ACS Synthetic …, 2024 - ACS Publications
Despite the wide use of plasmids in research and clinical production, the need to verify
plasmid sequences is a bottleneck that is too often underestimated in the manufacturing …

Identification of a common secondary mutation in the Neurospora crassa knockout collection conferring a cell fusion-defective phenotype

A Montenegro-Montero, A Goity, PF Canessa… - Microbiology …, 2023 - Am Soc Microbiol
Gene-deletion mutants represent a powerful tool to study gene function. The filamentous
fungus Neurospora crassa is a well-established model organism, and features a …

[HTML][HTML] Hybrid sequencing facilitates robust de novo plasmid assembly

SI Hernandez, CT Berezin, KM Miller, SJ Peccoud… - bioRxiv, 2024 - ncbi.nlm.nih.gov
Despite the wide use of plasmids in research and clinical production, the verification of
plasmid sequences is a bottleneck that is too often overlooked in the manufacturing process …

Discovery and characterization of a novel pathogen Erwinia pyri sp. nov. associated with pear dieback: taxonomic insights and genomic analysis

L He, R Huang, H Chen, L Zhao, Z Zhang - Frontiers in Microbiology, 2024 - frontiersin.org
In 2022, a novel disease similar to pear fire blight was found in a pear orchard in Zhangye
City, Gansu Province, China. The disease mainly damages the branches, leaves, and fruits …

CleanSeq: A pipeline for contamination detection, cleanup, and mutation verifications from microbial genome sequencing data

C Wang, Y Xia, Y Liu, C Kang, N Lu, D Tian, H Lu… - Applied Sciences, 2022 - mdpi.com
Contaminations frequently occur in bacterial cultures, which significantly affect the
reproducibility and reliability of the results from whole-genome sequencing (WGS) …

Potentials of pathogen research through the lens of cyberbiosecurity, or what threat actors can learn from the Covid-19 pandemic

S Mueller - Cyberbiosecurity: A New Field to Deal with Emerging …, 2023 - Springer
After 3+ years of investigation, the origin of SARS-CoV-2 remains unclear, and both a
spillover event from nature and a lab accident are heavily debated. As the Covid pandemic …

Genetic Analysis and Fine Mapping of QTL for the Erect Leaf in Mutant mths29 Induced through Fast Neutron in Wheat

Z Yang, J Gu, M Zhao, X Fan, H Guo, Y Xie, J Zhang… - Biology, 2024 - mdpi.com
Simple Summary Erect leaves are one of the important phenotypes for plants to adapt to
dense planting. This study obtained the erect leaf mutant mths29 through fast neutron …

Genetic interactions derived from high-throughput phenotyping of 6589 yeast cell cycle mutants

JE Gallegos, NR Adames, MF Rogers… - NPJ systems biology …, 2020 - nature.com
Over the last 30 years, computational biologists have developed increasingly realistic
mathematical models of the regulatory networks controlling the division of eukaryotic cells …

YPK9 and WHI2 Negatively Interact during Oxidative Stress

F Joseph, D Miller, OV Evgrafov, WJ Chirico - Microorganisms, 2021 - mdpi.com
Yeast PARK9 (YPK9) shares homology with human ATP13A2, which encodes a polyamine
transporter implicated in juvenile forms of Parkinson's disease. We used YPK9 to gain …

[PDF][PDF] YPK9 and WHI2 Negatively Interact during Oxidative Stress. Microorganisms 2021, 9, 2584

F Joseph, D Miller, OV Evgrafov, WJ Chirico - 2021 - researchgate.net
Yeast PARK9 (YPK9) shares homology with human ATP13A2, which encodes a polyamine
transporter implicated in juvenile forms of Parkinson's disease. We used YPK9 to gain …