DNA2 nuclease/helicase is a structure-specific nuclease, 5′-to-3′ helicase, and DNA-
dependent ATPase. It is involved in multiple DNA metabolic pathways, including Okazaki …

The perturbation of DNA replication, a phenomena termed “replication stress”, is a driving
force of genome instability and a hallmark of cancer cells. Among the DNA repair …

Pif1 DNA helicase is a potent unwinder of G-quadruplex (G4) structures in vitro and
functions to maintain genome stability at G4 sequences in Saccharomyces cerevisiae. Here …

Break-induced replication (BIR) is mutagenic, and thus its use requires tight regulation, yet
the underlying mechanisms remain elusive. Here we uncover an important role of 53BP1 in …

Efficient and faithful replication of the genome is essential to maintain genome stability.
Replication is carried out by a multiprotein complex called the replisome, which encounters …

Saccharomyces cerevisiae Pif1 is a multi-functional DNA helicase that plays diverse roles in
the maintenance of the nuclear and mitochondrial genomes. Two isoforms of Pif1 are …

Genome replication is frequently impeded by highly stable DNA secondary structures,
including G-quadruplex (G4) DNA, that can hinder the progression of the replication fork …

The two PIF1 family helicases in Saccharomyces cerevisiae, Rrm3, and ScPif1, associate
with thousands of sites throughout the genome where they perform overlapping and distinct …

Pif1 family helicases are multifunctional proteins conserved in eukaryotes, from yeast to
humans. They are important for the genome maintenance in both nuclei and mitochondria …

Members of the conserved Pif1 family of 5′-3′ DNA helicases can unwind G4s and
mitigate their negative impact on genome stability. In Saccharomyces cerevisiae, two Pif1 …