Fluorescent probes for lipid rafts: from model membranes to living cells

AS Klymchenko, R Kreder - Chemistry & biology, 2014 - cell.com
Membrane microdomains (rafts) remain one of the controversial issues in biophysics.
Fluorescent molecular probes, which make these lipid nanostructures visible through optical …

Molecular rotors: fluorescent sensors for microviscosity and conformation of biomolecules

M Paez‐Perez, MK Kuimova - Angewandte Chemie, 2024 - Wiley Online Library
The viscosity and crowding of biological environment are considered vital for the correct
cellular function, and alterations in these parameters are known to underly a number of …

Fatty acid membrane assembly on coacervate microdroplets as a step towards a hybrid protocell model

TY Dora Tang, C Rohaida Che Hak, AJ Thompson… - Nature …, 2014 - nature.com
Mechanisms of prebiotic compartmentalization are central to providing insights into how
protocellular systems emerged on the early Earth. Protocell models are based …

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria

JE Horne, DJ Brockwell, SE Radford - Journal of Biological Chemistry, 2020 - ASBMB
β-Barrel outer membrane proteins (OMPs) represent the major proteinaceous component of
the outer membrane (OM) of Gram-negative bacteria. These proteins perform key roles in …

Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density

J Steinkühler, E Sezgin, I Urbančič, C Eggeling… - Communications …, 2019 - nature.com
Regulation of plasma membrane curvature and composition governs essential cellular
processes. The material property of bending rigidity describes the energetic cost of …

A molecular rotor for measuring viscosity in plasma membranes of live cells

I López-Duarte, TT Vu, MA Izquierdo, JA Bull… - Chemical …, 2014 - pubs.rsc.org
Molecular rotors have emerged as versatile probes for microscopic viscosity in live cells,
however, the exclusive localisation of rotors in the plasma membrane has remained elusive …

Molecular mechanism of viscosity sensitivity in BODIPY rotors and application to motion-based fluorescent sensors

X Liu, W Chi, Q Qiao, SV Kokate, EP Cabrera, Z Xu… - Acs …, 2020 - ACS Publications
Viscosity in the intracellular microenvironment shows a significant difference in various
organelles and is closely related to cellular processes. Such microviscosity in live cells is …

Fluorescent flippers for mechanosensitive membrane probes

M Dal Molin, Q Verolet, A Colom, R Letrun… - Journal of the …, 2015 - ACS Publications
In this report,“fluorescent flippers” are introduced to create planarizable push–pull probes
with the mechanosensitivity and fluorescence lifetime needed for practical use in biology …

Redesigning solvatochromic probe laurdan for imaging lipid order selectively in cell plasma membranes

DI Danylchuk, E Sezgin, P Chabert… - Analytical …, 2020 - ACS Publications
Imaging of biological membranes by environmentally sensitive solvatochromic probes, such
as Laurdan, provides information about the organization of lipids, their ordering, and their …

Probing elastic and viscous properties of phospholipid bilayers using neutron spin echo spectroscopy

M Nagao, EG Kelley, R Ashkar… - The journal of …, 2017 - ACS Publications
The elastic and viscous properties of self-assembled amphiphilic membranes dictate the
intricate hierarchy of their structure and dynamics ranging from the diffusion of individual …