Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo,
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …

This article continues the series of Surveys and Summaries on restriction endonucleases
(REases) begun this year in Nucleic Acids Research. Here we discuss 'Type II'REases, the …

Proteins belonging to PD-(D/E) XK phosphodiesterases constitute a functionally diverse
superfamily with representatives involved in replication, restriction, DNA repair and tRNA …

DNA methylation-dependent restriction enzymes have many applications in genetic
engineering and in the analysis of the epigenetic state of eukaryotic genomes. Nevertheless …

The rise of antibiotic-resistant pathogens and the widespread occurrence of inflammatory
diseases have raised considerable global health concerns. Consequently, there is a critical …

Many restriction endonucleases are dimers that act symmetrically at palindromic DNA
sequences, with each active site cutting one strand. In contrast, FokI acts asymmetrically at a …

To explore the possibility of using restriction enzymes in a synthetic biology based on
artificially expanded genetic information systems (AEGIS), 24 type-II restriction …

Abstract Characterization and prediction of the DNA-biding regions in proteins are essential
for our understanding of how proteins recognize/bind DNA. We analyze the unbound (U) …

Restriction endonucleases naturally target DNA duplexes. Systematic screening has
identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes …

Endonucleases that generate DNA double strand breaks often employ two independent
subunits such that the active site from each subunit cuts either DNA strand. Restriction …