Combating antimicrobial resistance via single-cell diagnostic technologies powered by droplet microfluidics

K Hsieh, KE Mach, P Zhang, JC Liao… - Accounts of chemical …, 2021 - ACS Publications
Conspectus Antimicrobial resistance is a global threat that if left unchecked could lead to 10
million annual mortalities by 2050. One factor contributing to the rise of multi-drug-resistant …

MXene coupled with CRISPR-Cas12a for analysis of endotoxin and bacteria

A Sheng, P Wang, J Yang, L Tang, F Chen… - Analytical …, 2021 - ACS Publications
With hydrophilic surface and high density of functional groups, MXene can efficiently adsorb
single-stranded DNA to enhance target-induced strand release and quench the …

Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology

Y Ou, S Cao, J Zhang, W Dong, Z Yang, Z Yu - TrAC Trends in Analytical …, 2021 - Elsevier
Infectious diseases caused by pathogenic microbes have posed a major health issue for the
public, such as the ongoing COVID-19 global pandemic. In recent years, wastewater-based …

Portable integrated digital PCR system for the point-of-care quantification of BK virus from urine samples

L Xu, H Qu, DG Alonso, Z Yu, Y Yu, Y Shi, C Hu… - Biosensors and …, 2021 - Elsevier
This paper presents a portable integrated digital PCR (PI-dPCR) system with a self-
partitioning SlipChip (sp-SlipChip) microfluidic device for the quantitative analysis of BK …

High-throughput identification and quantification of single bacterial cells in the microbiota

J Jin, R Yamamoto, T Takeuchi, G Cui… - Nature …, 2022 - nature.com
The bacterial microbiota works as a community that consists of many individual organisms,
ie, cells. To fully understand the function of bacterial microbiota, individual cells must be …

ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

I Abellan-Schneyder, AJ Schusser, K Neuhaus - BMC microbiology, 2021 - Springer
Background One limiting factor of short amplicon 16S rRNA gene sequencing approaches is
the use of low DNA amounts in the amplicon generation step. Especially for low-biomass …

Detection of Shiga toxin-producing Escherichia coli (STEC) in beef products using droplet digital PCR

JA Capobianco, M Clark, A Cariou, A Leveau… - International journal of …, 2020 - Elsevier
Many of the current accredited methods for the molecular detection of Shiga toxin-producing
Escherichia coli (STEC) in foods rely on a PCR-based screen for the pathotype-specific …

Microfluidic one-pot digital droplet FISH using LNA/DNA molecular beacons for bacteria detection and absolute quantification

YT Kao, S Calabrese, N Borst, M Lehnert, YK Lai… - Biosensors, 2022 - mdpi.com
We demonstrate detection and quantification of bacterial load with a novel microfluidic one-
pot wash-free fluorescence in situ hybridization (FISH) assay in droplets. The method offers …

Single-tube multiplex digital polymerase chain reaction assay for molecular diagnosis and prediction of severity of spinal muscular atrophy

C Tan, Y Yan, N Guo, F Wang, S Wang, L Zhu… - Analytical …, 2022 - ACS Publications
Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disease
characterized by the degeneration of motor neurons and progressive muscle atrophy …

Drop it all: extraction-free detection of targeted marine species through optimized direct droplet digital PCR

M Scriver, U von Ammon, C Youngbull, X Pochon… - PeerJ, 2024 - peerj.com
Molecular biomonitoring programs increasingly use environmental DNA (eDNA) for
detecting targeted species such as marine non-indigenous species (NIS) or endangered …