Gene drives gaining speed
E Bier - Nature Reviews Genetics, 2022 - nature.com
Gene drives are selfish genetic elements that are transmitted to progeny at super-Mendelian
(> 50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are …
(> 50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are …
CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Bispecific antibodies targeting mutant RAS neoantigens
J Douglass, EHC Hsiue, BJ Mog, MS Hwang… - Science …, 2021 - science.org
Mutations in the RAS oncogenes occur in multiple cancers, and ways to target these
mutations has been the subject of intense research for decades. Most of these efforts are …
mutations has been the subject of intense research for decades. Most of these efforts are …
Interactions between nascent proteins translated by adjacent ribosomes drive homomer assembly
INTRODUCTION Most newly synthesized proteins associate into macromolecular
complexes to become functional. Complex formation requires that subunits find each other …
complexes to become functional. Complex formation requires that subunits find each other …
Direct optical measurement of intramolecular distances with angstrom precision
Optical investigations of nanometer distances between proteins, their subunits, or other
biomolecules have been the exclusive prerogative of Förster resonance energy transfer …
biomolecules have been the exclusive prerogative of Förster resonance energy transfer …
Preclinical studies show that Co-STARs combine the advantages of chimeric antigen and T cell receptors for the treatment of tumors with low antigen densities
BJ Mog, N Marcou, SR DiNapoli… - Science translational …, 2024 - science.org
Two types of engineered T cells have been successfully used to treat patients with cancer,
one with an antigen recognition domain derived from antibodies [chimeric antigen receptors …
one with an antigen recognition domain derived from antibodies [chimeric antigen receptors …
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
MS Schubert, B Thommandru, J Woodley, R Turk… - Scientific Reports, 2021 - nature.com
CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded
breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular …
breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular …
CRISPR/Cas9-mediated multi-allelic gene targeting in sugarcane confers herbicide tolerance
Sugarcane is the source of 80% of the sugar and 26% of the bioethanol produced globally.
However, its complex, highly polyploid genome (2 n= 100–120) impedes crop improvement …
However, its complex, highly polyploid genome (2 n= 100–120) impedes crop improvement …
Precise gene replacement in rice by RNA transcript-templated homologous recombination
One of the main obstacles to gene replacement in plants is efficient delivery of a donor
repair template (DRT) into the nucleus for homology-directed DNA repair (HDR) of double …
repair template (DRT) into the nucleus for homology-directed DNA repair (HDR) of double …
An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
O Kanca, J Zirin, J Garcia-Marques, SM Knight… - Elife, 2019 - elifesciences.org
We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila
genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic …
genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic …