Critical review of 16S rRNA gene sequencing workflow in microbiome studies: From primer selection to advanced data analysis
A Regueira‐Iglesias, C Balsa‐Castro… - Molecular Oral …, 2023 - Wiley Online Library
The multi‐batch reanalysis approach of jointly reevaluating gene/genome sequences from
different works has gained particular relevance in the literature in recent years. The large …
different works has gained particular relevance in the literature in recent years. The large …
The salivary microbiome as a diagnostic biomarker of periodontitis: a 16S multi-batch study before and after the removal of batch effects
A Regueira-Iglesias, B Suárez-Rodríguez… - Frontiers in Cellular …, 2024 - frontiersin.org
Introduction Microbiome-based clinical applications that improve diagnosis related to oral
health are of great interest to precision dentistry. Predictive studies on the salivary …
health are of great interest to precision dentistry. Predictive studies on the salivary …
Impact of 16S rRNA gene redundancy and primer pair selection on the quantification and classification of oral microbiota in next-generation sequencing
A Regueira-Iglesias, L Vázquez-González… - Microbiology …, 2023 - Am Soc Microbiol
This study aimed to evaluate the number of 16S rRNA genes in the complete genomes of
the bacterial and archaeal species inhabiting the human mouth and to assess how the use …
the bacterial and archaeal species inhabiting the human mouth and to assess how the use …
Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and database
T Nagai, T Shiba, K Komatsu, T Watanabe… - Microbiology …, 2024 - journals.asm.org
ABSTRACT 16S rRNA gene amplicon sequencing analysis is used to investigate bacterial
communities; however, the estimated bacterial composition can differ from the original due …
communities; however, the estimated bacterial composition can differ from the original due …
The salivary microbiome as a diagnostic biomarker of health and periodontitis: a large-scale meta-omics analysis before and after the removal of batch effects
A Regueira-Iglesias, T Blanco-Pintos, M Relvas… - 2023 - researchsquare.com
Background Methodological differences in 16S rRNA sequencing studies have significant
effects on the diversity of the results obtained, making comparisons in the form of traditional …
effects on the diversity of the results obtained, making comparisons in the form of traditional …