Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

[HTML][HTML] Live-cell imaging in the deep learning era

JW Pylvänäinen, E Gómez-de-Mariscal… - Current Opinion in Cell …, 2023 - Elsevier
Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy

B Yang, M Lange, A Millett-Sikking, X Zhao… - Nature …, 2022 - nature.com
The promise of single-objective light-sheet microscopy is to combine the convenience of
standard single-objective microscopes with the speed, coverage, resolution and gentleness …

Light-sheets and smart microscopy, an exciting future is dawning

S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Isotropic imaging across spatial scales with axially swept light-sheet microscopy

KM Dean, T Chakraborty, S Daetwyler, J Lin… - Nature protocols, 2022 - nature.com
Light-sheet fluorescence microscopy is a rapidly growing technique that has gained
tremendous popularity in the life sciences owing to its high-spatiotemporal resolution and …

Roadmap on digital holography-based quantitative phase imaging

V Balasubramani, M Kujawińska, C Allier, V Anand… - Journal of …, 2021 - mdpi.com
Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or
technical microstructures, merging beneficial features identified with microscopy …

Open microscopy in the life sciences: quo vadis?

J Hohlbein, B Diederich, B Marsikova, EG Reynaud… - Nature …, 2022 - nature.com
Light microscopy enables researchers to observe cellular mechanisms with high spatial and
temporal resolution. However, the increasing complexity of current imaging technologies …

A hitchhiker's guide through the bio‐image analysis software universe

R Haase, E Fazeli, D Legland, M Doube, S Culley… - Febs …, 2022 - Wiley Online Library
Modern research in the life sciences is unthinkable without computational methods for
extracting, quantifying and visualising information derived from microscopy imaging data of …

Scanless two-photon voltage imaging

RR Sims, I Bendifallah, C Grimm… - Nature …, 2024 - nature.com
Two-photon voltage imaging has long been heralded as a transformative approach capable
of answering many long-standing questions in modern neuroscience. However, exploiting …

Live cell microscopy: From image to insight

AP Cuny, FP Schlottmann, JC Ewald, S Pelet… - Biophysics …, 2022 - pubs.aip.org
Live-cell microscopy is a powerful tool that can reveal cellular behavior as well as the
underlying molecular processes. A key advantage of microscopy is that by visualizing …