Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
KC Gwosch, JK Pape, F Balzarotti, P Hoess… - Nature …, 2020 - nature.com
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
Deep learning enables fast and dense single-molecule localization with high accuracy
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …
cellular structures with nanometer resolution, but standard analysis algorithms require …
α-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson's disease
Protein aggregation causes α-synuclein to switch from its physiological role to a pathological
toxic gain of function. Under physiological conditions, monomeric α-synuclein improves ATP …
toxic gain of function. Under physiological conditions, monomeric α-synuclein improves ATP …
Parameter-free image resolution estimation based on decorrelation analysis
A Descloux, KS Grußmayer, A Radenovic - Nature methods, 2019 - nature.com
Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …
Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
Despite significant progress, high-speed live-cell super-resolution studies remain limited to
specialized optical setups, generally requiring intense phototoxic illumination. Here, we …
specialized optical setups, generally requiring intense phototoxic illumination. Here, we …
Quantitative evaluation of software packages for single-molecule localization microscopy
The quality of super-resolution images obtained by single-molecule localization microscopy
(SMLM) depends largely on the software used to detect and accurately localize point …
(SMLM) depends largely on the software used to detect and accurately localize point …
Precisely and accurately localizing single emitters in fluorescence microscopy
H Deschout, FC Zanacchi, M Mlodzianoski, A Diaspro… - Nature …, 2014 - nature.com
Methods based on single-molecule localization and photophysics have brought nanoscale
imaging with visible light into reach. This has enabled single-particle tracking applications …
imaging with visible light into reach. This has enabled single-particle tracking applications …
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary
objective to improve the resolution, field of view, and usability over previous variants. Owing …
objective to improve the resolution, field of view, and usability over previous variants. Owing …