Delayed diagnosis of cancer using conventional diagnostic modalities needs to be
addressed to reduce the mortality rate of cancer. Recently, 2D nanomaterial-enabled …

The past decade has seen great strides in our understanding of the genetic basis of human
disease. Arguably, the most profound impact has been in the area of cancer genetics, where …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

Nucleic acid diagnostics is dominated by fluorescence-based assays that use complex and
expensive enzyme-based target or signal-amplification procedures,,,,,. Many clinical …

Background: In most measurements of gene expression, mRNA is first reverse-transcribed
into cDNA. We studied the reverse transcription reaction and its consequences for …

This paper describes a SlipChip to perform digital PCR in a very simple and inexpensive
format. The fluidic path for introducing the sample combined with the PCR mixture was …

Biomarkers are essential tools in clinical research and practice. Useful biomarkers must
combine good measurability, validated association with biological processes or outcomes …

Real-time reverse transcriptase polymerase chain reaction (RT-PCR) is becoming a widely
used method to quantify cytokines from cells, tissues, or tissue biopsies. The method allows …

The characterization of the diversity of species living within ecosystems is of major scientific
interest to understand the functioning of these ecosystems. It is also becoming a societal …

Diagnosis of infectious diseases suffers from long turnaround times for gold standard culture-
based identification of bacterial pathogens, therefore impeding timely specific antimicrobial …