Aminoglycosides have been an essential component of the armamentarium in the treatment
of life-threatening infections. Unfortunately, their efficacy has been reduced by the surge and …

The three classes of enzymes which inactivate aminoglycosides and lead to bacterial
resistance are reviewed. DNA hybridization studies have shown that different genes can …

The conserved transcriptional regulator heat shock factor 1 (Hsf1) is a key sensor of
proteotoxic and other stress in the eukaryotic cytosol. We surveyed Hsf1 activity in a genome …

MITOCHONDRIAL encephalomyopathies are usually divided into three distinct clinical
subgroups:(1) mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like …

Dominant drug resistance markers are commonly used genetic tools in fungi other than
Saccharomyces cerevisiae (Austin et al., 1990; Avalos et al., 1989; Chakraborty et al., 1991) …

YIP vectors Transplacement Integration into reiterated DNA Transposition vectors
Transcriptional promoters and terminators Foreign versus yeast promoters Glycolytic …

We report the development of an advanced system for transfer and expression of exogenous
genes in mammalian cells based on Moloney murine leukemia virus (Mo MuLV). Extensive …

The low rate of homologous recombination exhibited by wild-type strains of filamentous
fungi has hindered development of high-throughput gene knockout procedures for this …

Aminoglycoside antibiotics have had a major impact on our ability to treat bacterial infections
for the past half century. Whereas the interest in these versatile antibiotics continues to be …

Yeasts are widely used in production of recombinant proteins of medical or industrial
interest. For each individual product, the most suitable expression system has to be …