Strategies for achieving high-level expression of genes in Escherichia coli

SC Makrides - Microbiological reviews, 1996 - Am Soc Microbiol
Progress in our understanding of several biological processes promises to broaden the
usefulness of Escherichia coli as a tool for gene expression. There is an expanding choice …

DNA binding sites: representation and discovery

GD Stormo - Bioinformatics, 2000 - academic.oup.com
The purpose of this article is to provide a brief history of the development and application of
computer algorithms for the analysis and prediction of DNA binding sites. This problem can …

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

LM Guzman, D Belin, MJ Carson… - Journal of …, 1995 - Am Soc Microbiol
We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD
promoter of the araBAD (arabinose) operon and the gene encoding the positive and …

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

DB Smith, KS Johnson - Gene, 1988 - Elsevier
Plasmid expression vectors have been constructed that direct the synthesis of foreign
polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione …

RNA-peptide fusions for the in vitro selection of peptides and proteins

RW Roberts, JW Szostak - Proceedings of the National …, 1997 - National Acad Sciences
Covalent fusions between an mRNA and the peptide or protein that it encodes can be
generated by in vitro translation of synthetic mRNAs that carry puromycin, a peptidyl …

The mitochondrial DNA molecule ofDrosophila yakuba: Nucleotide sequence, gene organization, and genetic code

DO Clary, DR Wolstenholme - Journal of Molecular Evolution, 1985 - Springer
The sequence of the 16,019 nucleotide-pair mitochondrial DNA (mtDNA) molecule of
Drosophila yakuba is presented. This molecule contains the genes for two rRNAs, 22 tRNAs …

[图书][B] Bioinformatics: the machine learning approach

P Baldi, S Brunak - 2001 - books.google.com
A guide to machine learning approaches and their application to the analysis of biological
data. An unprecedented wealth of data is being generated by genome sequencing projects …

A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

ER LaVallie, EA DiBlasio, S Kovacic, KL Grant… - Bio/technology, 1993 - nature.com
We have developed a versatile Escherichia coli expression system based on the use of E.
coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated …

Nomenclature for incompletely specified bases in nucleic acid sequences: recommendations 1984.

A Cornish-Bowden - Nucleic acids research, 1985 - ncbi.nlm.nih.gov
With the introduction of methods of rapid nucleic acid sequence determination, synthesis of
mixed oligonucleotide probes and computer-assisted analysis of nucleic acid sequences …

Sequence and gene organization of the chicken mitochondrial genome: a novel gene order in higher vertebrates

P Desjardins, R Morais - Journal of molecular biology, 1990 - Elsevier
Abstract The 16,775 base-pair mitochondrial genome of the white Leghorn chicken has
been cloned and sequenced. The avian genome encodes the same set of genes (13 …