[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022 - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

Identifying biases and their potential solutions in human microbiome studies

JT Nearing, AM Comeau, MGI Langille - Microbiome, 2021 - Springer
Advances in DNA sequencing technology have vastly improved the ability of researchers to
explore the microbial inhabitants of the human body. Unfortunately, while these studies have …

Ancient and modern environmental DNA

MW Pedersen… - … of the Royal …, 2015 - royalsocietypublishing.org
DNA obtained from environmental samples such as sediments, ice or water (environmental
DNA, eDNA), represents an important source of information on past and present biodiversity …

Evaluation of methods for the extraction and purification of DNA from the human microbiome

S Yuan, DB Cohen, J Ravel, Z Abdo, LJ Forney - PloS one, 2012 - journals.plos.org
Background DNA extraction is an essential step in all cultivation-independent approaches to
characterize microbial diversity, including that associated with the human body. A …

Comparative analysis of fecal DNA extraction methods with phylogenetic microarray: effective recovery of bacterial and archaeal DNA using mechanical cell lysis

A Salonen, J Nikkilä, J Jalanka-Tuovinen… - Journal of …, 2010 - Elsevier
Several different protocols are used for fecal DNA extraction, which is an integral step in all
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …

Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phoD phosphorus mineraliser group in pasture soils

H Tan, M Barret, MJ Mooij, O Rice, JP Morrissey… - Biology and Fertility of …, 2013 - Springer
Fertilisers, especially nitrogen (N) and phosphorus (P) supplies, are frequently used in
agricultural soil management to attain high crop yields. However, the intensive application of …

Environmental DNA metabarcoding studies are critically affected by substrate selection

A Koziol, M Stat, T Simpson, S Jarman… - Molecular Ecology …, 2019 - Wiley Online Library
Effective biomonitoring is critical for driving management outcomes that ensure long‐term
sustainability of the marine environment. In recent years, environmental DNA (eDNA) …

How to get more out of molecular fingerprints: practical tools for microbial ecology

M Marzorati, L Wittebolle, N Boon… - Environmental …, 2008 - Wiley Online Library
Community‐level molecular techniques are widely used in comparative microbial ecology to
assess the diversity of microbial communities and their response to changing environments …

Accessing the soil metagenome for studies of microbial diversity

TO Delmont, P Robe, S Cecillon, IM Clark… - Applied and …, 2011 - Am Soc Microbiol
Soil microbial communities contain the highest level of prokaryotic diversity of any
environment, and metagenomic approaches involving the extraction of DNA from soil can …

Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences

B Wagner Mackenzie, DW Waite… - Frontiers in microbiology, 2015 - frontiersin.org
The human gut contains dense and diverse microbial communities which have profound
influences on human health. Gaining meaningful insights into these communities requires …