While the Staudinger reaction has first been described a hundred years ago in 1919, the
ligation reaction became one of the most important and efficient bioconjugation techniques …

Bioorthogonal chemistries can be used to tag diverse classes of biomolecules in cells and
other complex environments. With over 20 unique transformations now available, though …

The expression of cell surface glycans terminating with sialic acid (SA) residues has been
found to correlate with various disease states there among cancer. We here report a novel …

Metabolic glycoengineering (MGE) is a technique for manipulating cellular metabolism to
modulate glycosylation. MGE is used to increase the levels of natural glycans and, more …

As the science of immunology grows increasingly mechanistic, motivation for developing
quantitative, design-based engineering approaches has also evolved, both for therapeutic …

Bioorthogonal phosphines were introduced in the context of the Staudinger ligation over 20
years ago. Since that time, phosphine probes have been used in myriad applications to tag …

Tumor-targeting strategies for nanoparticles have been predominantly based on
optimization of physical properties or conjugation with biological ligands. However, their …

Tumor cells overexpress surface markers which are absent from normal cells. These tumor-
restricted antigenic signatures are a fundamental basis for distinguishing on-target from off …

In metabolic glycoengineering (MGE), cells or animals are treated with unnatural derivatives
of monosaccharides. After entering the cytosol, these sugar analogues are metabolized and …

Radioimmunotherapy (RIT) of solid tumors is hampered by low tumor-to-nontumor (T/NT)
ratios of the radiolabeled monoclonal antibodies resulting in low tumor doses in patients …