Mitochondria are derived from eubacteria; however, in most eukaryotes, novel mechanisms
for the propagation of this organelle and its genome have evolved. This review focuses on …

Although fusion mechanisms are highly conserved in evolution and among organelles of the
exocytic and endocytic pathways, yeast vacuole homotypic fusion offers unique technical …

We have used a lipophilic styryl dye, N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-
hexatrienyl) pyridinium dibromide (FM 4-64), as a vital stain to follow bulk membrane …

The Cdc6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at
origins of DNA replication in the budding yeast Saccharomyces cerevisiae. This reaction is …

S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and
tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic …

The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of
exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron …

Faithful inheritance of genetic information requires that DNA be copied only once each cell
cycle. Initiation of DNA replication involves the establishment of a prereplication complex …

Protein transport in eukaryotic cells requires the selective docking and fusion of transport
intermediates with the appropriate target membrane. t-SNARE molecules that are …

The basic reaction mechanisms for membrane fusion in the trafficking of intracellular
membranes and in exocytosis are probably identical. But in contrast to regulated exocytosis …

Delivery of proteins to the vacuole of the yeast Saccharomyces cerevisiae provides an
excellent model system in which to study vacuole and lysosome biogenesis and membrane …