Tutorial: guidance for quantitative confocal microscopy

J Jonkman, CM Brown, GD Wright, KI Anderson… - Nature protocols, 2020 - nature.com
When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …

Best practices and tools for reporting reproducible fluorescence microscopy methods

P Montero Llopis, RA Senft, TJ Ross-Elliott… - Nature …, 2021 - nature.com
Although fluorescence microscopy is ubiquitous in biomedical research, microscopy
methods reporting is inconsistent and perhaps undervalued. We emphasize the importance …

Expansion microscopy

F Chen, PW Tillberg, ES Boyden - Science, 2015 - science.org
In optical microscopy, fine structural details are resolved by using refraction to magnify
images of a specimen. We discovered that by synthesizing a swellable polymer network …

Neuronal morphometry directly from bitmap images

TA Ferreira, AV Blackman, J Oyrer, S Jayabal… - Nature …, 2014 - nature.com
Neuroscientists measure the tree-like structures of neurons in order to better understand
how neural circuits are constructed and how neural information is processed. In 1953 …

A pipeline for multidimensional confocal analysis of mitochondrial morphology, function, and dynamics in pancreatic β-cells

A Chaudhry, R Shi, DS Luciani - American Journal of …, 2020 - journals.physiology.org
Live-cell imaging of mitochondrial function and dynamics can provide vital insights into both
physiology and pathophysiology, including of metabolic diseases like type 2 diabetes …

[HTML][HTML] Any way you slice it—a comparison of confocal microscopy techniques

J Jonkman, CM Brown - Journal of biomolecular techniques: JBT, 2015 - ncbi.nlm.nih.gov
The confocal fluorescence microscope has become a popular tool for life sciences
researchers, primarily because of its ability to remove blur from outside of the focal plane of …

High numerical aperture Fourier ptychography: principle, implementation and characterization

X Ou, R Horstmeyer, G Zheng, C Yang - Optics express, 2015 - opg.optica.org
Fourier ptychography (FP) utilizes illumination control and computational post-processing to
increase the resolution of bright-field microscopes. In effect, FP extends the fixed numerical …

Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms

JW Krieger, AP Singh, N Bag, CS Garbe… - Nature protocols, 2015 - nature.com
Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …

Technical considerations in the Verasonics research ultrasound platform for developing a photoacoustic imaging system

K Kratkiewicz, R Manwar, Y Zhou… - Biomedical Optics …, 2021 - opg.optica.org
Photoacoustic imaging (PAI) is an emerging functional and molecular imaging technology
that has attracted much attention in the past decade. Recently, many researchers have used …

Replication-transcription switch in human mitochondria

K Agaronyan, YI Morozov, M Anikin, D Temiakov - Science, 2015 - science.org
Coordinated replication and expression of the mitochondrial genome is critical for
metabolically active cells during various stages of development. However, it is not known …