Anionic phospholipids control mechanisms of GPCR-G protein recognition

N Thakur, AP Ray, L Sharp, B Jin, A Duong… - Nature …, 2023 - nature.com
G protein-coupled receptors (GPCRs) are embedded in phospholipids that strongly
influence drug-stimulated signaling. Anionic lipids are particularly important for GPCR …

Advances in nanodisc platforms for membrane protein purification

JM Pettersen, Y Yang, AS Robinson - Trends in Biotechnology, 2023 - cell.com
Membrane scaffold protein nanodiscs (MSPNDs) are an invaluable tool for improving
purified membrane protein (MP) stability and activity compared to traditional micellar …

Dual mechanisms of cholesterol-GPCR interactions that depend on membrane phospholipid composition

AP Ray, N Thakur, NG Pour, MT Eddy - Structure, 2023 - cell.com
Cholesterol is a critical component of mammalian cell membranes and an allosteric
modulator of G protein-coupled receptors (GPCRs), but divergent views exist on the …

Single-molecule visualization of human A2A adenosine receptor activation by a G protein and constitutively activating mutations

S Wei, NG Pour, S Tiruvadi-Krishnan, AP Ray… - Communications …, 2023 - nature.com
Mutations that constitutively activate G protein-coupled receptors (GPCRs), known as
constitutively activating mutations (CAMs), modify cell signaling and interfere with drugs …

Single-molecule analysis reveals that a glucagon-bound extracellular domain of the glucagon receptor is dynamic

T Liu, S Khanal, GD Hertslet, R Lamichhane - Journal of Biological …, 2023 - ASBMB
Dynamic information is vital to understanding the activation mechanism of G protein-coupled
receptors (GPCRs). Despite the availability of high-resolution structures of different …

Global insights into the fine tuning of human A2AAR conformational dynamics in a ternary complex with an engineered G protein viewed by NMR

G Ferré, K Anazia, LO Silva, N Thakur, AP Ray… - Cell reports, 2022 - cell.com
G protein-coupled receptor (GPCR) conformational plasticity enables formation of ternary
signaling complexes with intracellular proteins in response to binding extracellular ligands …

[HTML][HTML] Membrane mimetic-dependence of GPCR energy landscapes

N Thakur, AP Ray, B Jin, NP Afsharian, E Lyman… - Structure, 2024 - cell.com
We leveraged variable-temperature 19 F-NMR spectroscopy to compare the conformational
equilibria of the human A 2A adenosine receptor (A 2A AR), a class AG protein-coupled …

Functional GPCR expression in eukaryotic LEXSY system

A Luginina, I Maslov, P Khorn, O Volkov… - Journal of Molecular …, 2023 - Elsevier
G protein-coupled receptors (GPCRs) form the largest superfamily of membrane proteins in
the human genome, and represent one of the most important classes of drug targets. Their …

Exploring GPCR conformational dynamics using single-molecule fluorescence

E Agyemang, AN Gonneville, S Tiruvadi-Krishnan… - Methods, 2024 - Elsevier
G protein-coupled receptors (GPCRs) are membrane proteins that transmit specific external
stimuli into cells by changing their conformation. This conformational change allows them to …

Biophysical Dissection of Isolated GPCRs: The Adenosine A2A Receptor under the Bistouries

JL Banères, T Botzanowski, JA Boutin, B Calamini… - Receptors, 2023 - mdpi.com
In an effort to provide an overview of the biophysical approaches used to study G-protein-
coupled receptors, we chose to consider the adenosine A2A receptor as a model, as it is …