In this review, we highlight the current understanding of translation elongation and recoding
in eukaryotes. In addition to providing an overview of the process, recent advances in our …

This work summarizes our current understanding of the elongation and termination/recycling
phases of eukaryotic protein synthesis. We focus here on recent advances in the field. In …

Primary cilia organize Hedgehog signaling and shape embryonic development, and their
dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic …

Hsp70s comprise a deeply conserved chaperone family that has a central role in
maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 …

Pseudomonas exotoxin A (PE) is a highly toxic protein secreted by the opportunistic
pathogen Pseudomonas aeruginosa. The modular structure and corresponding mechanism …

Recombinant immunotoxins are fusion proteins that contain the cytotoxic portion of a protein
toxin fused to the Fv portion of an antibody. The Fv binds to an antigen on a target cell and …

Translocation into the endoplasmic reticulum (ER) is an initial and crucial biogenesis step
for all secreted and endomembrane proteins in eukaryotes. ER insertion can take place …

Diphthamide (DPH), a conserved amino acid modification on eukaryotic translation
elongation factor eEF2, is synthesized via a complex, multi-enzyme pathway. While DPH is …

Transfer RNA (tRNA) is subject to a multitude of posttranscriptional modifications which can
profoundly impact its functionality as the essential adaptor molecule in messenger RNA …

The components of the cellular protein translation machinery, such as ribosomal proteins
and translation factors, are subject to numerous post-translational modifications. In …