Recombinant spider silk: promises and bottlenecks

M Ramezaniaghdam, ND Nahdi… - Frontiers in Bioengineering …, 2022 - frontiersin.org
Spider silk threads have exceptional mechanical properties such as toughness, elasticity
and low density, which reach maximum values compared to other fibre materials. They are …

Silk-based delivery systems of bioactive molecules

K Numata, DL Kaplan - Advanced drug delivery reviews, 2010 - Elsevier
Silks are biodegradable, biocompatible, self-assembling proteins that can also be tailored
via genetic engineering to contain specific chemical features, offering utility for drug and …

Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering

G Jin, MP Prabhakaran, S Ramakrishna - Acta biomaterialia, 2011 - Elsevier
Bone marrow (BM) mesenchymal stem cells (MSC) capable of differentiating along the
epidermal lineage on engineered nanofibrous scaffolds have great potential for …

Spider silk proteins: recent advances in recombinant production, structure–function relationships and biomedical applications

A Rising, M Widhe, J Johansson… - Cellular and Molecular …, 2011 - Springer
Spider dragline silk is an outstanding material made up of unique proteins—spidroins.
Analysis of the amino acid sequences of full-length spidroins reveals a tripartite composition …

Delivering on the promise of recombinant silk-inspired proteins for drug delivery

C Gonzalez-Obeso, EJ Hartzell, RA Scheel… - Advanced drug delivery …, 2023 - Elsevier
Effective drug delivery is essential for the success of a medical treatment. Polymeric drug
delivery systems (DDSs) are preferred over systemic administration of drugs due to their …

[HTML][HTML] Production of protein-based polymers in Pichia pastoris

MWT Werten, G Eggink, MAC Stuart, FA de Wolf - Biotechnology advances, 2019 - Elsevier
Materials science and genetic engineering have joined forces over the last three decades in
the development of so-called protein-based polymers. These are proteins, typically with …

Recombinant production of spider silk proteins

A Heidebrecht, T Scheibel - Advances in applied microbiology, 2013 - Elsevier
Natural spider silk fibers combine extraordinary properties such as stability and flexibility
which results in a toughness superseding that of all other fiber materials. As the spider's …

Artificial skin–culturing of different skin cell lines for generating an artificial skin substitute on cross-weaved spider silk fibres

H Wendt, A Hillmer, K Reimers, JW Kuhbier… - PloS one, 2011 - journals.plos.org
Background In the field of Plastic Reconstructive Surgery the development of new innovative
matrices for skin repair is in urgent need. The ideal biomaterial should promote attachment …

Tissue engineered neural constructs composed of neural precursor cells, recombinant spidroin and PRP for neural tissue regeneration

VP Baklaushev, VG Bogush, VA Kalsin… - Scientific Reports, 2019 - nature.com
We have designed a novel two-component matrix (SPRPix) for the encapsulation of directly
reprogrammed human neural precursor cells (drNPC). The matrix is comprised of 1) a solid …

To spin or not to spin: spider silk fibers and more

E Doblhofer, A Heidebrecht, T Scheibel - Applied microbiology and …, 2015 - Springer
Spider silk fibers have a sophisticated hierarchical structure composed of proteins with
highly repetitive sequences. Their extraordinary mechanical properties, defined by a unique …