Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Integrating microfluidics and synthetic biology: advancements and diverse applications across organisms

C Leal-Alves, Z Deng, N Kermeci, SCC Shih - Lab on a Chip, 2024 - pubs.rsc.org
Synthetic biology is the design and modification of biological systems for specific functions,
integrating several disciplines like engineering, genetics, and computer science. The field of …

Smart droplet microfluidic system for single-cell selective lysis and real-time sorting based on microinjection and image recognition

Z Yu, J Jin, S Chen, L Shui, H Chen, L Shi… - Analytical …, 2023 - ACS Publications
Single-cell analysis has important implications for understanding the specificity of cells. To
analyze the specificity of rare cells in complex blood and biopsy samples, selective lysis of …

Ultrahigh-Throughput Single Emulsion Droplet Screening for the Discovery of New B Antigen Cleaving Enzymes

C Olagnon, JF Wardman, F Liu, HM Chen, H Moon… - ACS …, 2024 - ACS Publications
In search of efficient α-galactosidases that can convert B red blood cells (RBCs) to universal
type RBCs, we have developed a simple and robust system for ultrahigh-throughput droplet …

Durlobactam to boost the clinical utility of standard of care β-lactams against Mycobacterium abscessus lung disease

DA Negatu, WW Aragaw, TT Gebresilase… - Antimicrobial Agents …, 2024 - journals.asm.org
ABSTRACT β-Lactams present several desirable pharmacodynamic features leading to the
rapid eradication of many bacterial pathogens. Imipenem (IPM) and cefoxitin (FOX) are …

A Coupled Ketoreductase‐Diaphorase Assay for the Detection of Polyethylene Terephthalate‐Hydrolyzing Activity

M Gimeno‐Pérez, JD Finnigan, C Echeverria… - …, 2022 - Wiley Online Library
In the last two decades, several PET‐degrading enzymes from already known
microorganisms or metagenomic sources have been discovered to face the growing …

Vector redesign and in‐droplet cell‐growth improves enrichment and recovery in live Escherichia coli

BDG Eenink, TS Kaminski… - Microbial …, 2022 - Wiley Online Library
Directed evolution (DE) is a widely used method for improving the function of biomolecules
via multiple rounds of mutation and selection. Microfluidic droplets have emerged as an …

Electrogenetic communication structures to encode, propagate, and decode information through synthetic biology

ES VanArsdale - 2022 - search.proquest.com
Cellular communication, the exchange of information between cells, is a process in which
cues are encoded, propagated, and decoded through shared chemical and physical …

[引用][C] 若干裂解基因盒子的表征及应用

付生伟, 金帆 - 生物工程学报, 2023