State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Crossing the blood–brain barrier in primates is a major obstacle for gene delivery to the
brain. Adeno-associated viruses (AAVs) promise robust, non-invasive gene delivery from the …

The vascular interface of the brain, known as the blood–brain barrier (BBB), is understood to
maintain brain function in part via its low transcellular permeability,–. Yet, recent studies …

Parkinson's disease is a synucleinopathy that is characterized by motor dysfunction, death of
midbrain dopaminergic neurons and accumulation of α-synuclein (α-Syn) aggregates …

Abstract Analysis of entire transparent rodent bodies after clearing could provide holistic
biological information in health and disease, but reliable imaging and quantification of …

Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Modern optogenetics can be tuned to evoke activity that corresponds to naturally occurring
local or global activity in timing, magnitude or individual-cell patterning. This outcome has …

Recombinant adeno-associated viruses (rAAVs) are commonly used vehicles for in vivo
gene transfer 1, 2, 3, 4, 5, 6. However, the tropism repertoire of naturally occurring AAVs is …

Identifying the spatial organization of tissues at cellular resolution from single-cell gene
expression profiles is essential to understanding biological systems. Using an in situ 3D …