The role of RNA polymerase (Pol) III in eukaryotic transcription is commonly thought of as
being restricted to a small set of highly expressed, housekeeping non–protein-coding (nc) …

Gene transcription in the cell nucleus is a complex and highly regulated process.
Transcription in eukaryotes requires three distinct RNA polymerases, each of which employs …

Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and
CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease …

We present Cas-Designer, a user-friendly program to aid researchers in choosing
appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided …

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently
been developed. However, especially when multiple genetic manipulations are required …

Eukaryotic cells execute complex transcriptional programs in which specific loci throughout
the genome are regulated in distinct ways by targeted regulatory assemblies. We have …

Here, we report the design, construction, and characterization of a tRNA neochromosome, a
designer chromosome that functions as an additional, de novo counterpart to the native …

As the adaptor that decodes mRNA sequence into protein, the basic aspects of tRNA
structure and function are central to all studies of biology. Yet the complexities of their …

Small nucleolar RNAs (snoRNAs) are one of the most ancient and numerous families of non-
protein-coding RNAs (ncRNAs). The main function of snoRNAs–to guide site-specific rRNA …

Our understanding of how genotype controls phenotype is limited by the scale at which we
can precisely alter the genome and assess the phenotypic consequences of each …